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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phymatum Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP462282BXT
CSB-EP462282BXT
CSB-BP462282BXT
CSB-MP462282BXT
CSB-EP462282BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L34 (rpmH) CSB-YP462283BXT
CSB-EP462283BXT
CSB-BP462283BXT
CSB-MP462283BXT
CSB-EP462283BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP462284BXT
CSB-EP462284BXT
CSB-BP462284BXT
CSB-MP462284BXT
CSB-EP462284BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum ATP-dependent protease subunit HslV (hslV) CSB-YP462285BXT
CSB-EP462285BXT
CSB-BP462285BXT
CSB-MP462285BXT
CSB-EP462285BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Arginine--tRNA ligase (argS), partial CSB-YP462286BXT
CSB-EP462286BXT
CSB-BP462286BXT
CSB-MP462286BXT
CSB-EP462286BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Acetaldehyde dehydrogenase 1 (Bphy_3511) CSB-YP462287BXT
CSB-EP462287BXT
CSB-BP462287BXT
CSB-MP462287BXT
CSB-EP462287BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ferredoxin--NADP reductase (Bphy_3740) CSB-YP462288BXT
CSB-EP462288BXT
CSB-BP462288BXT
CSB-MP462288BXT
CSB-EP462288BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Homoserine kinase (thrB) CSB-YP462289BXT
CSB-EP462289BXT
CSB-BP462289BXT
CSB-MP462289BXT
CSB-EP462289BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Betaine aldehyde dehydrogenase (betB) CSB-YP462290BXT
CSB-EP462290BXT
CSB-BP462290BXT
CSB-MP462290BXT
CSB-EP462290BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DNA replication and repair protein recF (recF) CSB-YP462291YAW
CSB-EP462291YAW
CSB-BP462291YAW
CSB-MP462291YAW
CSB-EP462291YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP462292YAW
CSB-EP462292YAW
CSB-BP462292YAW
CSB-MP462292YAW
CSB-EP462292YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Chromosome partition protein MukB (mukB), partial CSB-YP462293YAW
CSB-EP462293YAW
CSB-BP462293YAW
CSB-MP462293YAW
CSB-EP462293YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein Xni (xni) CSB-YP462294YAW
CSB-EP462294YAW
CSB-BP462294YAW
CSB-MP462294YAW
CSB-EP462294YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP462295YAW
CSB-EP462295YAW
CSB-BP462295YAW
CSB-MP462295YAW
CSB-EP462295YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Dihydroxy-acid dehydratase (ilvD), partial CSB-YP462296YAW
CSB-EP462296YAW
CSB-BP462296YAW
CSB-MP462296YAW
CSB-EP462296YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP462297YAW
CSB-EP462297YAW
CSB-BP462297YAW
CSB-MP462297YAW
CSB-EP462297YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 4-alpha-L-fucosyltransferase (wecF) CSB-YP462298YAW
CSB-EP462298YAW
CSB-BP462298YAW
CSB-MP462298YAW
CSB-EP462298YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein CyaY (cyaY) CSB-YP462299YAW
CSB-EP462299YAW
CSB-BP462299YAW
CSB-MP462299YAW
CSB-EP462299YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP462300YAW
CSB-EP462300YAW
CSB-BP462300YAW
CSB-MP462300YAW
CSB-EP462300YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB tRNA-modifying protein ygfZ (YPTS_3305) CSB-YP462301YAW
CSB-EP462301YAW
CSB-BP462301YAW
CSB-MP462301YAW
CSB-EP462301YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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