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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Mitochondrial inner membrane organizing system protein 1 (Minos1), partial CSB-YP462402RA1
CSB-EP462402RA1
CSB-BP462402RA1
CSB-MP462402RA1
CSB-EP462402RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Carbon storage regulator homolog (csrA) CSB-YP462403BUJ
CSB-EP462403BUJ
CSB-BP462403BUJ
CSB-MP462403BUJ
CSB-EP462403BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Elongation factor P (efp) CSB-YP462404BUJ
CSB-EP462404BUJ
CSB-BP462404BUJ
CSB-MP462404BUJ
CSB-EP462404BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP462405BUJ
CSB-EP462405BUJ
CSB-BP462405BUJ
CSB-MP462405BUJ
CSB-EP462405BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 30S ribosomal protein S12 (rpsL) CSB-YP462406BUJ
CSB-EP462406BUJ
CSB-BP462406BUJ
CSB-MP462406BUJ
CSB-EP462406BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 50S ribosomal protein L34 (rpmH) CSB-YP462407BUJ
CSB-EP462407BUJ
CSB-BP462407BUJ
CSB-MP462407BUJ
CSB-EP462407BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Lipoprotein signal peptidase (lspA), partial CSB-YP462408BUJ1
CSB-EP462408BUJ1
CSB-BP462408BUJ1
CSB-MP462408BUJ1
CSB-EP462408BUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 50S ribosomal protein L16 (rplP) CSB-YP462409BUJ
CSB-EP462409BUJ
CSB-BP462409BUJ
CSB-MP462409BUJ
CSB-EP462409BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 50S ribosomal protein L5 (rplE) CSB-YP462410BUJ
CSB-EP462410BUJ
CSB-BP462410BUJ
CSB-MP462410BUJ
CSB-EP462410BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 50S ribosomal protein L17 (rplQ) CSB-YP462411BUJ
CSB-EP462411BUJ
CSB-BP462411BUJ
CSB-MP462411BUJ
CSB-EP462411BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Methionine--tRNA ligase (metG), partial CSB-YP462412BUJ
CSB-EP462412BUJ
CSB-BP462412BUJ
CSB-MP462412BUJ
CSB-EP462412BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 30S ribosomal protein S4 (rpsD) CSB-YP462413BUJ
CSB-EP462413BUJ
CSB-BP462413BUJ
CSB-MP462413BUJ
CSB-EP462413BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Ribonuclease Z (rnz) CSB-YP462414BUJ
CSB-EP462414BUJ
CSB-BP462414BUJ
CSB-MP462414BUJ
CSB-EP462414BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Ribosome-binding factor A (rbfA) CSB-YP462415BUJ
CSB-EP462415BUJ
CSB-BP462415BUJ
CSB-MP462415BUJ
CSB-EP462415BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Probable transcriptional regulatory protein BH0025 (BH0025) CSB-YP462416BUJ
CSB-EP462416BUJ
CSB-BP462416BUJ
CSB-MP462416BUJ
CSB-EP462416BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Probable rRNA maturation factor (BH0060) CSB-YP462417BUJ
CSB-EP462417BUJ
CSB-BP462417BUJ
CSB-MP462417BUJ
CSB-EP462417BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 50S ribosomal protein L9 (rplI) CSB-YP462418TPS
CSB-EP462418TPS
CSB-BP462418TPS
CSB-MP462418TPS
CSB-EP462418TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum UvrABC system protein B (uvrB), partial CSB-YP462419TPS
CSB-EP462419TPS
CSB-BP462419TPS
CSB-MP462419TPS
CSB-EP462419TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 30S ribosomal protein S10 (rpsJ) CSB-YP462420TPS
CSB-EP462420TPS
CSB-BP462420TPS
CSB-MP462420TPS
CSB-EP462420TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 30S ribosomal protein S3 (rpsC) CSB-YP462421TPS
CSB-EP462421TPS
CSB-BP462421TPS
CSB-MP462421TPS
CSB-EP462421TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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