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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia phytofirmans Protein TolB (tolB) CSB-YP462542BXU
CSB-EP462542BXU
CSB-BP462542BXU
CSB-MP462542BXU
CSB-EP462542BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Urease subunit gamma (ureA) CSB-YP462543BXU
CSB-EP462543BXU
CSB-BP462543BXU
CSB-MP462543BXU
CSB-EP462543BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Probable septum site-determining protein MinC (minC) CSB-YP462544BXU
CSB-EP462544BXU
CSB-BP462544BXU
CSB-MP462544BXU
CSB-EP462544BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP462545BXU
CSB-EP462545BXU
CSB-BP462545BXU
CSB-MP462545BXU
CSB-EP462545BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP462546BXU
CSB-EP462546BXU
CSB-BP462546BXU
CSB-MP462546BXU
CSB-EP462546BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0176 protein Bphyt_1252 (Bphyt_1252) CSB-YP462547BXU
CSB-EP462547BXU
CSB-BP462547BXU
CSB-MP462547BXU
CSB-EP462547BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Probable transcriptional regulatory protein Bphyt_1301 (Bphyt_1301) CSB-YP462548BXU
CSB-EP462548BXU
CSB-BP462548BXU
CSB-MP462548BXU
CSB-EP462548BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP462549BXU
CSB-EP462549BXU
CSB-BP462549BXU
CSB-MP462549BXU
CSB-EP462549BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosome-binding factor A (rbfA) CSB-YP462550BXU
CSB-EP462550BXU
CSB-BP462550BXU
CSB-MP462550BXU
CSB-EP462550BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S6 (rpsF) CSB-YP462551BXU
CSB-EP462551BXU
CSB-BP462551BXU
CSB-MP462551BXU
CSB-EP462551BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Recombination protein RecR (recR) CSB-YP462552BXU
CSB-EP462552BXU
CSB-BP462552BXU
CSB-MP462552BXU
CSB-EP462552BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP462553BXU
CSB-EP462553BXU
CSB-BP462553BXU
CSB-MP462553BXU
CSB-EP462553BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Putative phosphotransferase Bphyt_2439 (Bphyt_2439) CSB-YP462554BXU
CSB-EP462554BXU
CSB-BP462554BXU
CSB-MP462554BXU
CSB-EP462554BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP462555BXU
CSB-EP462555BXU
CSB-BP462555BXU
CSB-MP462555BXU
CSB-EP462555BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Probable Fe (2+)-trafficking protein CSB-YP462556BXU
CSB-EP462556BXU
CSB-BP462556BXU
CSB-MP462556BXU
CSB-EP462556BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosome maturation factor RimM (rimM) CSB-YP462557BXU
CSB-EP462557BXU
CSB-BP462557BXU
CSB-MP462557BXU
CSB-EP462557BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Integration host factor subunit beta (ihfB) CSB-YP462558BXU
CSB-EP462558BXU
CSB-BP462558BXU
CSB-MP462558BXU
CSB-EP462558BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP462559BXU
CSB-EP462559BXU
CSB-BP462559BXU
CSB-MP462559BXU
CSB-EP462559BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD), partial CSB-YP462560BXU
CSB-EP462560BXU
CSB-BP462560BXU
CSB-MP462560BXU
CSB-EP462560BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP462561BXU
CSB-EP462561BXU
CSB-BP462561BXU
CSB-MP462561BXU
CSB-EP462561BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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