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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP462602DUG
CSB-EP462602DUG
CSB-BP462602DUG
CSB-MP462602DUG
CSB-EP462602DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ureidoglycolate hydrolase (allA) CSB-YP462603SYZ
CSB-EP462603SYZ
CSB-BP462603SYZ
CSB-MP462603SYZ
CSB-EP462603SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein CrcB homolog (crcB), partial CSB-YP462604SYZ1
CSB-EP462604SYZ1
CSB-BP462604SYZ1
CSB-MP462604SYZ1
CSB-EP462604SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP462605SYZ
CSB-EP462605SYZ
CSB-BP462605SYZ
CSB-MP462605SYZ
CSB-EP462605SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Methylglyoxal synthase (mgsA) CSB-YP462606SYZ
CSB-EP462606SYZ
CSB-BP462606SYZ
CSB-MP462606SYZ
CSB-EP462606SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP462607SYZ
CSB-EP462607SYZ
CSB-BP462607SYZ
CSB-MP462607SYZ
CSB-EP462607SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative ECA polymerase (wzyE), partial CSB-YP462608SYZ
CSB-EP462608SYZ
CSB-BP462608SYZ
CSB-MP462608SYZ
CSB-EP462608SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 D-ribose pyranase (rbsD) CSB-YP462609SYZ
CSB-EP462609SYZ
CSB-BP462609SYZ
CSB-MP462609SYZ
CSB-EP462609SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP462610SYZ
CSB-EP462610SYZ
CSB-BP462610SYZ
CSB-MP462610SYZ
CSB-EP462610SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP462611SYZ
CSB-EP462611SYZ
CSB-BP462611SYZ
CSB-MP462611SYZ
CSB-EP462611SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP462612SYZ
CSB-EP462612SYZ
CSB-BP462612SYZ
CSB-MP462612SYZ
CSB-EP462612SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP462613SYZ
CSB-EP462613SYZ
CSB-BP462613SYZ
CSB-MP462613SYZ
CSB-EP462613SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP462614SYZ
CSB-EP462614SYZ
CSB-BP462614SYZ
CSB-MP462614SYZ
CSB-EP462614SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 ATP synthase epsilon chain (atpC) CSB-YP462615SYZ
CSB-EP462615SYZ
CSB-BP462615SYZ
CSB-MP462615SYZ
CSB-EP462615SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Membrane protein insertase YidC (yidC), partial CSB-YP462616SYZ1
CSB-EP462616SYZ1
CSB-BP462616SYZ1
CSB-MP462616SYZ1
CSB-EP462616SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein CyaY (cyaY) CSB-YP462617SYZ
CSB-EP462617SYZ
CSB-BP462617SYZ
CSB-MP462617SYZ
CSB-EP462617SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable malate:quinone oxidoreductase (mqo), partial CSB-YP462618SYZ
CSB-EP462618SYZ
CSB-BP462618SYZ
CSB-MP462618SYZ
CSB-EP462618SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 PKHD-type hydroxylase ybiX (ybiX) CSB-YP462619SYZ
CSB-EP462619SYZ
CSB-BP462619SYZ
CSB-MP462619SYZ
CSB-EP462619SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP462620SYZ
CSB-EP462620SYZ
CSB-BP462620SYZ
CSB-MP462620SYZ
CSB-EP462620SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0761 membrane protein yihY (yihY), partial CSB-YP462621SYZ1
CSB-EP462621SYZ1
CSB-BP462621SYZ1
CSB-MP462621SYZ1
CSB-EP462621SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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