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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Chlorobium limicola Probable rRNA maturation factor (Clim_1583) CSB-YP463002DSR
CSB-EP463002DSR
CSB-BP463002DSR
CSB-MP463002DSR
CSB-EP463002DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola S-adenosylmethionine synthase (metK) CSB-YP463003DSR
CSB-EP463003DSR
CSB-BP463003DSR
CSB-MP463003DSR
CSB-EP463003DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Heat-inducible transcription repressor HrcA (hrcA) CSB-YP463004DSR
CSB-EP463004DSR
CSB-BP463004DSR
CSB-MP463004DSR
CSB-EP463004DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP463005DSR
CSB-EP463005DSR
CSB-BP463005DSR
CSB-MP463005DSR
CSB-EP463005DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola DNA mismatch repair protein MutS (mutS), partial CSB-YP463006DSR
CSB-EP463006DSR
CSB-BP463006DSR
CSB-MP463006DSR
CSB-EP463006DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Aspartate carbamoyltransferase (pyrB) CSB-YP463007DSR
CSB-EP463007DSR
CSB-BP463007DSR
CSB-MP463007DSR
CSB-EP463007DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP463008DSR
CSB-EP463008DSR
CSB-BP463008DSR
CSB-MP463008DSR
CSB-EP463008DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP463009DSR
CSB-EP463009DSR
CSB-BP463009DSR
CSB-MP463009DSR
CSB-EP463009DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Putative nickel-responsive regulator (Clim_2003) CSB-YP463010DSR
CSB-EP463010DSR
CSB-BP463010DSR
CSB-MP463010DSR
CSB-EP463010DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola UvrABC system protein B (uvrB), partial CSB-YP463011DSR
CSB-EP463011DSR
CSB-BP463011DSR
CSB-MP463011DSR
CSB-EP463011DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Probable transcriptional regulatory protein Clim_0475 (Clim_0475) CSB-YP463012DSR
CSB-EP463012DSR
CSB-BP463012DSR
CSB-MP463012DSR
CSB-EP463012DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 60 kDa chaperonin (groL), partial CSB-YP463013DSR
CSB-EP463013DSR
CSB-BP463013DSR
CSB-MP463013DSR
CSB-EP463013DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP463014DSR
CSB-EP463014DSR
CSB-BP463014DSR
CSB-MP463014DSR
CSB-EP463014DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP463015DSR
CSB-EP463015DSR
CSB-BP463015DSR
CSB-MP463015DSR
CSB-EP463015DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S11 (rpsK) CSB-YP463016DSR
CSB-EP463016DSR
CSB-BP463016DSR
CSB-MP463016DSR
CSB-EP463016DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S3 (rpsC) CSB-YP463017DSR
CSB-EP463017DSR
CSB-BP463017DSR
CSB-MP463017DSR
CSB-EP463017DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Pantothenate synthetase (panC) CSB-YP463018DSR
CSB-EP463018DSR
CSB-BP463018DSR
CSB-MP463018DSR
CSB-EP463018DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola GTPase obg (obg) CSB-YP463019DSR
CSB-EP463019DSR
CSB-BP463019DSR
CSB-MP463019DSR
CSB-EP463019DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP463020DSR1
CSB-EP463020DSR1
CSB-BP463020DSR1
CSB-MP463020DSR1
CSB-EP463020DSR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola UPF0133 protein Clim_0875 (Clim_0875) CSB-YP463021DSR
CSB-EP463021DSR
CSB-BP463021DSR
CSB-MP463021DSR
CSB-EP463021DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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