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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum 50S ribosomal protein L18 (rplR) CSB-YP462582DUG
CSB-EP462582DUG
CSB-BP462582DUG
CSB-MP462582DUG
CSB-EP462582DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable butyrate kinase (buk) CSB-YP462583DUG
CSB-EP462583DUG
CSB-BP462583DUG
CSB-MP462583DUG
CSB-EP462583DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Threonine--tRNA ligase (thrS), partial CSB-YP462584DUG
CSB-EP462584DUG
CSB-BP462584DUG
CSB-MP462584DUG
CSB-EP462584DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA ligase (ligA), partial CSB-YP462585DUG
CSB-EP462585DUG
CSB-BP462585DUG
CSB-MP462585DUG
CSB-EP462585DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S16 (rpsP) CSB-YP462586DUG
CSB-EP462586DUG
CSB-BP462586DUG
CSB-MP462586DUG
CSB-EP462586DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein RecA (recA) CSB-YP462587DUG
CSB-EP462587DUG
CSB-BP462587DUG
CSB-MP462587DUG
CSB-EP462587DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L31 (rpmE) CSB-YP462588DUG
CSB-EP462588DUG
CSB-BP462588DUG
CSB-MP462588DUG
CSB-EP462588DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine synthase (metK) CSB-YP462589DUG
CSB-EP462589DUG
CSB-BP462589DUG
CSB-MP462589DUG
CSB-EP462589DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D-ribose pyranase (rbsD) CSB-YP462590DUG
CSB-EP462590DUG
CSB-BP462590DUG
CSB-MP462590DUG
CSB-EP462590DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable endonuclease 4 (nfo) CSB-YP462591DUG
CSB-EP462591DUG
CSB-BP462591DUG
CSB-MP462591DUG
CSB-EP462591DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S20 (rpsT) CSB-YP462592DUG
CSB-EP462592DUG
CSB-BP462592DUG
CSB-MP462592DUG
CSB-EP462592DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Chaperone protein DnaJ (dnaJ) CSB-YP462593DUG
CSB-EP462593DUG
CSB-BP462593DUG
CSB-MP462593DUG
CSB-EP462593DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Tryptophan synthase beta chain (trpB) CSB-YP462594DUG
CSB-EP462594DUG
CSB-BP462594DUG
CSB-MP462594DUG
CSB-EP462594DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Regulatory protein recX (recX) CSB-YP462595DUG
CSB-EP462595DUG
CSB-BP462595DUG
CSB-MP462595DUG
CSB-EP462595DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Deoxycytidine triphosphate deaminase (dcd) CSB-YP462596DUG
CSB-EP462596DUG
CSB-BP462596DUG
CSB-MP462596DUG
CSB-EP462596DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Homoserine kinase (thrB) CSB-YP462597DUG
CSB-EP462597DUG
CSB-BP462597DUG
CSB-MP462597DUG
CSB-EP462597DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cobyric acid synthase (cobQ), partial CSB-YP462598DUG
CSB-EP462598DUG
CSB-BP462598DUG
CSB-MP462598DUG
CSB-EP462598DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UvrABC system protein B (uvrB), partial CSB-YP462599DUG
CSB-EP462599DUG
CSB-BP462599DUG
CSB-MP462599DUG
CSB-EP462599DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP462600DUG
CSB-EP462600DUG
CSB-BP462600DUG
CSB-MP462600DUG
CSB-EP462600DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum FMN-dependent NADH-azoreductase (azoR) CSB-YP462601DUG
CSB-EP462601DUG
CSB-BP462601DUG
CSB-MP462601DUG
CSB-EP462601DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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