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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylacidiphilum infernorum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP462962MSV
CSB-EP462962MSV
CSB-BP462962MSV
CSB-MP462962MSV
CSB-EP462962MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum 2-isopropylmalate synthase (leuA), partial CSB-YP462963MSV
CSB-EP462963MSV
CSB-BP462963MSV
CSB-MP462963MSV
CSB-EP462963MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP462964MSV
CSB-EP462964MSV
CSB-BP462964MSV
CSB-MP462964MSV
CSB-EP462964MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum ATP-dependent zinc metalloprotease FtsH 2 (ftsH2), partial CSB-YP462965MSV
CSB-EP462965MSV
CSB-BP462965MSV
CSB-MP462965MSV
CSB-EP462965MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (glgE), partial CSB-YP462966MSV
CSB-EP462966MSV
CSB-BP462966MSV
CSB-MP462966MSV
CSB-EP462966MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum 30S ribosomal protein S19 (rpsS) CSB-YP462967MSV
CSB-EP462967MSV
CSB-BP462967MSV
CSB-MP462967MSV
CSB-EP462967MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP462968MSV
CSB-EP462968MSV
CSB-BP462968MSV
CSB-MP462968MSV
CSB-EP462968MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP462969MSV
CSB-EP462969MSV
CSB-BP462969MSV
CSB-MP462969MSV
CSB-EP462969MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Probable transaldolase (tal) CSB-YP462970GFO
CSB-EP462970GFO
CSB-BP462970GFO
CSB-MP462970GFO
CSB-EP462970GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Trigger factor (tig) CSB-YP462971GFO
CSB-EP462971GFO
CSB-BP462971GFO
CSB-MP462971GFO
CSB-EP462971GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L21 (rplU) CSB-YP462972GFO
CSB-EP462972GFO
CSB-BP462972GFO
CSB-MP462972GFO
CSB-EP462972GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP462973GFO
CSB-EP462973GFO
CSB-BP462973GFO
CSB-MP462973GFO
CSB-EP462973GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi GTPase Der (der) CSB-YP462974GFO
CSB-EP462974GFO
CSB-BP462974GFO
CSB-MP462974GFO
CSB-EP462974GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Lipid-A-disaccharide synthase (lpxB) CSB-YP462975GFO
CSB-EP462975GFO
CSB-BP462975GFO
CSB-MP462975GFO
CSB-EP462975GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP462976GFO
CSB-EP462976GFO
CSB-BP462976GFO
CSB-MP462976GFO
CSB-EP462976GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Pantothenate synthetase (panC) CSB-YP462977GFO
CSB-EP462977GFO
CSB-BP462977GFO
CSB-MP462977GFO
CSB-EP462977GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Anthranilate phosphoribosyltransferase (trpD) CSB-YP462978GFO
CSB-EP462978GFO
CSB-BP462978GFO
CSB-MP462978GFO
CSB-EP462978GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi DNA mismatch repair protein mutL (mutL), partial CSB-YP462979GFO
CSB-EP462979GFO
CSB-BP462979GFO
CSB-MP462979GFO
CSB-EP462979GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S21 (rpsU) CSB-YP462980GFO
CSB-EP462980GFO
CSB-BP462980GFO
CSB-MP462980GFO
CSB-EP462980GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP462981GFO
CSB-EP462981GFO
CSB-BP462981GFO
CSB-MP462981GFO
CSB-EP462981GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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