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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter lovleyi Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP462982GFO
CSB-EP462982GFO
CSB-BP462982GFO
CSB-MP462982GFO
CSB-EP462982GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L9 (rplI) CSB-YP462983GFO
CSB-EP462983GFO
CSB-BP462983GFO
CSB-MP462983GFO
CSB-EP462983GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi tRNA (mo5U34)-methyltransferase CSB-YP462984GFO
CSB-EP462984GFO
CSB-BP462984GFO
CSB-MP462984GFO
CSB-EP462984GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP462985GFO
CSB-EP462985GFO
CSB-BP462985GFO
CSB-MP462985GFO
CSB-EP462985GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP462986GFO
CSB-EP462986GFO
CSB-BP462986GFO
CSB-MP462986GFO
CSB-EP462986GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L10 (rplJ) CSB-YP462987GFO
CSB-EP462987GFO
CSB-BP462987GFO
CSB-MP462987GFO
CSB-EP462987GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L23 (rplW) CSB-YP462988GFO
CSB-EP462988GFO
CSB-BP462988GFO
CSB-MP462988GFO
CSB-EP462988GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S17 (rpsQ) CSB-YP462989GFO
CSB-EP462989GFO
CSB-BP462989GFO
CSB-MP462989GFO
CSB-EP462989GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Adenylate kinase (adk) CSB-YP462990GFO
CSB-EP462990GFO
CSB-BP462990GFO
CSB-MP462990GFO
CSB-EP462990GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Dihydrodipicolinate reductase (dapB) CSB-YP462991GFO
CSB-EP462991GFO
CSB-BP462991GFO
CSB-MP462991GFO
CSB-EP462991GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP462992GFO
CSB-EP462992GFO
CSB-BP462992GFO
CSB-MP462992GFO
CSB-EP462992GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Elongation factor 4 (lepA), partial CSB-YP462993GFO
CSB-EP462993GFO
CSB-BP462993GFO
CSB-MP462993GFO
CSB-EP462993GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Regulatory protein recX (recX) CSB-YP462994GFO
CSB-EP462994GFO
CSB-BP462994GFO
CSB-MP462994GFO
CSB-EP462994GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP462995GFO
CSB-EP462995GFO
CSB-BP462995GFO
CSB-MP462995GFO
CSB-EP462995GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Ribosome maturation factor RimP (rimP) CSB-YP462996GFO
CSB-EP462996GFO
CSB-BP462996GFO
CSB-MP462996GFO
CSB-EP462996GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP462997GFO
CSB-EP462997GFO
CSB-BP462997GFO
CSB-MP462997GFO
CSB-EP462997GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Flagellar L-ring protein (flgH) CSB-YP462998GFO
CSB-EP462998GFO
CSB-BP462998GFO
CSB-MP462998GFO
CSB-EP462998GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola ATP-dependent protease subunit HslV (hslV) CSB-YP462999DSR
CSB-EP462999DSR
CSB-BP462999DSR
CSB-MP462999DSR
CSB-EP462999DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Large-conductance mechanosensitive channel (mscL), partial CSB-YP463000DSR1
CSB-EP463000DSR1
CSB-BP463000DSR1
CSB-MP463000DSR1
CSB-EP463000DSR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Adenylate kinase (adk) CSB-YP463001DSR
CSB-EP463001DSR
CSB-BP463001DSR
CSB-MP463001DSR
CSB-EP463001DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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