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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobium limicola Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP463022DSR
CSB-EP463022DSR
CSB-BP463022DSR
CSB-MP463022DSR
CSB-EP463022DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Ribosome maturation factor RimM (rimM) CSB-YP463023DSS
CSB-EP463023DSS
CSB-BP463023DSS
CSB-MP463023DSS
CSB-EP463023DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides ATP-dependent protease subunit HslV (hslV) CSB-YP463024DSS
CSB-EP463024DSS
CSB-BP463024DSS
CSB-MP463024DSS
CSB-EP463024DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Cobalamin synthase (cobS), partial CSB-YP463025DSS1
CSB-EP463025DSS1
CSB-BP463025DSS1
CSB-MP463025DSS1
CSB-EP463025DSS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP463026DSS1
CSB-EP463026DSS1
CSB-BP463026DSS1
CSB-MP463026DSS1
CSB-EP463026DSS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L32 (rpmF) CSB-YP463027DSS
CSB-EP463027DSS
CSB-BP463027DSS
CSB-MP463027DSS
CSB-EP463027DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Ferredoxin--NADP reductase (Cphamn1_1733) CSB-YP463028DSS
CSB-EP463028DSS
CSB-BP463028DSS
CSB-MP463028DSS
CSB-EP463028DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L11 (rplK) CSB-YP463029DSS
CSB-EP463029DSS
CSB-BP463029DSS
CSB-MP463029DSS
CSB-EP463029DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Large-conductance mechanosensitive channel (mscL), partial CSB-YP463030DSS1
CSB-EP463030DSS1
CSB-BP463030DSS1
CSB-MP463030DSS1
CSB-EP463030DSS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Lipoprotein signal peptidase (lspA), partial CSB-YP463031DSS1
CSB-EP463031DSS1
CSB-BP463031DSS1
CSB-MP463031DSS1
CSB-EP463031DSS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Aspartate 1-decarboxylase (panD) CSB-YP463032DSS
CSB-EP463032DSS
CSB-BP463032DSS
CSB-MP463032DSS
CSB-EP463032DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Probable endonuclease 4 (nfo) CSB-YP463033DSS
CSB-EP463033DSS
CSB-BP463033DSS
CSB-MP463033DSS
CSB-EP463033DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides HPr kinase/phosphorylase (hprK) CSB-YP463034DSS
CSB-EP463034DSS
CSB-BP463034DSS
CSB-MP463034DSS
CSB-EP463034DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Ribonuclease H (rnhA) CSB-YP463035DSS
CSB-EP463035DSS
CSB-BP463035DSS
CSB-MP463035DSS
CSB-EP463035DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L31 (rpmE) CSB-YP463036DSS
CSB-EP463036DSS
CSB-BP463036DSS
CSB-MP463036DSS
CSB-EP463036DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S21 (rpsU) CSB-YP463037DSS
CSB-EP463037DSS
CSB-BP463037DSS
CSB-MP463037DSS
CSB-EP463037DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 7-cyano-7-deazaguanine synthase (queC) CSB-YP463038DSS
CSB-EP463038DSS
CSB-BP463038DSS
CSB-MP463038DSS
CSB-EP463038DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Probable GTP-binding protein EngB (engB) CSB-YP463039DSS
CSB-EP463039DSS
CSB-BP463039DSS
CSB-MP463039DSS
CSB-EP463039DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L24 (rplX) CSB-YP463040DSS
CSB-EP463040DSS
CSB-BP463040DSS
CSB-MP463040DSS
CSB-EP463040DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L4 (rplD) CSB-YP463041DSS
CSB-EP463041DSS
CSB-BP463041DSS
CSB-MP463041DSS
CSB-EP463041DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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