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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L3 (rplC) CSB-YP463082AXI
CSB-EP463082AXI
CSB-BP463082AXI
CSB-MP463082AXI
CSB-EP463082AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L16 (rplP) CSB-YP463083AXI
CSB-EP463083AXI
CSB-BP463083AXI
CSB-MP463083AXI
CSB-EP463083AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L6 (rplF) CSB-YP463084AXI
CSB-EP463084AXI
CSB-BP463084AXI
CSB-MP463084AXI
CSB-EP463084AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosome maturation factor RimM (rimM) CSB-YP463085AXI
CSB-EP463085AXI
CSB-BP463085AXI
CSB-MP463085AXI
CSB-EP463085AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Tyrosine recombinase XerC (xerC) CSB-YP463086AXI
CSB-EP463086AXI
CSB-BP463086AXI
CSB-MP463086AXI
CSB-EP463086AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-dehydroquinate dehydratase (aroQ) CSB-YP463087AXI
CSB-EP463087AXI
CSB-BP463087AXI
CSB-MP463087AXI
CSB-EP463087AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP463088AXI
CSB-EP463088AXI
CSB-BP463088AXI
CSB-MP463088AXI
CSB-EP463088AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Peptide chain release factor 3 (prfC), partial CSB-YP463089AXI
CSB-EP463089AXI
CSB-BP463089AXI
CSB-MP463089AXI
CSB-EP463089AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Thiosulfate sulfurtransferase glpE (glpE) CSB-YP463090AXI
CSB-EP463090AXI
CSB-BP463090AXI
CSB-MP463090AXI
CSB-EP463090AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP463091AXI
CSB-EP463091AXI
CSB-BP463091AXI
CSB-MP463091AXI
CSB-EP463091AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP463092AXI
CSB-EP463092AXI
CSB-BP463092AXI
CSB-MP463092AXI
CSB-EP463092AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UvrABC system protein C (uvrC), partial CSB-YP463093AXI
CSB-EP463093AXI
CSB-BP463093AXI
CSB-MP463093AXI
CSB-EP463093AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Methionine--tRNA ligase (metG), partial CSB-YP463094AXI
CSB-EP463094AXI
CSB-BP463094AXI
CSB-MP463094AXI
CSB-EP463094AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glutamyl-tRNA reductase (hemA) CSB-YP463095AXI
CSB-EP463095AXI
CSB-BP463095AXI
CSB-MP463095AXI
CSB-EP463095AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0246 protein APP7_0648 (APP7_0648) CSB-YP463096AXI
CSB-EP463096AXI
CSB-BP463096AXI
CSB-MP463096AXI
CSB-EP463096AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Carbon storage regulator homolog (csrA) CSB-YP463097AXI
CSB-EP463097AXI
CSB-BP463097AXI
CSB-MP463097AXI
CSB-EP463097AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Cell division protein FtsB (ftsB), partial CSB-YP463098AXI
CSB-EP463098AXI
CSB-BP463098AXI
CSB-MP463098AXI
CSB-EP463098AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP463099AXI
CSB-EP463099AXI
CSB-BP463099AXI
CSB-MP463099AXI
CSB-EP463099AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Deoxycytidine triphosphate deaminase (dcd) CSB-YP463100AXI
CSB-EP463100AXI
CSB-BP463100AXI
CSB-MP463100AXI
CSB-EP463100AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Leucine--tRNA ligase (leuS), partial CSB-YP463101AXI
CSB-EP463101AXI
CSB-BP463101AXI
CSB-MP463101AXI
CSB-EP463101AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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