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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O157:H7 Uncharacterized MFS-type transporter ycaD (ycaD), partial CSB-YP466783EOE
CSB-EP466783EOE
CSB-BP466783EOE
CSB-MP466783EOE
CSB-EP466783EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase L (rlmL) CSB-YP466784EOE
CSB-EP466784EOE
CSB-BP466784EOE
CSB-MP466784EOE
CSB-EP466784EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Methylglyoxal synthase (mgsA) CSB-YP466785EOE
CSB-EP466785EOE
CSB-BP466785EOE
CSB-MP466785EOE
CSB-EP466785EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L30 (rpmD) CSB-YP466786EOE
CSB-EP466786EOE
CSB-BP466786EOE
CSB-MP466786EOE
CSB-EP466786EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L5 (rplE) CSB-YP466787EOE
CSB-EP466787EOE
CSB-BP466787EOE
CSB-MP466787EOE
CSB-EP466787EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L22 (rplV) CSB-YP466788EOE
CSB-EP466788EOE
CSB-BP466788EOE
CSB-MP466788EOE
CSB-EP466788EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0270 protein YheU (yheU) CSB-YP466789EOE
CSB-EP466789EOE
CSB-BP466789EOE
CSB-MP466789EOE
CSB-EP466789EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP466790EOE
CSB-EP466790EOE
CSB-BP466790EOE
CSB-MP466790EOE
CSB-EP466790EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Chaperone modulatory protein CbpM (cbpM) CSB-YP466791EOE
CSB-EP466791EOE
CSB-BP466791EOE
CSB-MP466791EOE
CSB-EP466791EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) CSB-YP466792EOE
CSB-EP466792EOE
CSB-BP466792EOE
CSB-MP466792EOE
CSB-EP466792EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Deoxycytidine triphosphate deaminase (dcd) CSB-YP466793EOE
CSB-EP466793EOE
CSB-BP466793EOE
CSB-MP466793EOE
CSB-EP466793EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP466794EOE
CSB-EP466794EOE
CSB-BP466794EOE
CSB-MP466794EOE
CSB-EP466794EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP466795EOE
CSB-EP466795EOE
CSB-BP466795EOE
CSB-MP466795EOE
CSB-EP466795EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable lipid kinase YegS (yegS) CSB-YP466796EOE
CSB-EP466796EOE
CSB-BP466796EOE
CSB-MP466796EOE
CSB-EP466796EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Methionine--tRNA ligase (metG) CSB-YP466797EOE
CSB-EP466797EOE
CSB-BP466797EOE
CSB-MP466797EOE
CSB-EP466797EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable phosphatase YcdX (ycdX) CSB-YP466798EOE
CSB-EP466798EOE
CSB-BP466798EOE
CSB-MP466798EOE
CSB-EP466798EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L32 (rpmF) CSB-YP466799EOE
CSB-EP466799EOE
CSB-BP466799EOE
CSB-MP466799EOE
CSB-EP466799EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0758 protein yicR (yicR) CSB-YP466800EOE
CSB-EP466800EOE
CSB-BP466800EOE
CSB-MP466800EOE
CSB-EP466800EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA ligase B (ligB) CSB-YP466801EOE
CSB-EP466801EOE
CSB-BP466801EOE
CSB-MP466801EOE
CSB-EP466801EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Pyrimidine-specific ribonucleoside hydrolase rihB (rihB) CSB-YP466802EOE
CSB-EP466802EOE
CSB-BP466802EOE
CSB-MP466802EOE
CSB-EP466802EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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