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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae 50S ribosomal protein L28 (rpmB) CSB-YP468963KBH
CSB-EP468963KBH
CSB-BP468963KBH
CSB-MP468963KBH
CSB-EP468963KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP468964KBH
CSB-EP468964KBH
CSB-BP468964KBH
CSB-MP468964KBH
CSB-EP468964KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP468965KBH
CSB-EP468965KBH
CSB-BP468965KBH
CSB-MP468965KBH
CSB-EP468965KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 3-dehydroquinate synthase (aroB) CSB-YP468966KBH
CSB-EP468966KBH
CSB-BP468966KBH
CSB-MP468966KBH
CSB-EP468966KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP468967KBH
CSB-EP468967KBH
CSB-BP468967KBH
CSB-MP468967KBH
CSB-EP468967KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Urease subunit alpha (ureC), partial CSB-YP468968KBH
CSB-EP468968KBH
CSB-BP468968KBH
CSB-MP468968KBH
CSB-EP468968KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Bifunctional protein HldE (hldE) CSB-YP468969KBH
CSB-EP468969KBH
CSB-BP468969KBH
CSB-MP468969KBH
CSB-EP468969KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Putative Holliday junction resolvase (KPK_0727) CSB-YP468970KBH
CSB-EP468970KBH
CSB-BP468970KBH
CSB-MP468970KBH
CSB-EP468970KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphoglycerate kinase (pgk) CSB-YP468971KBH
CSB-EP468971KBH
CSB-BP468971KBH
CSB-MP468971KBH
CSB-EP468971KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0267 protein KPK_0770 (KPK_0770) CSB-YP468972KBH
CSB-EP468972KBH
CSB-BP468972KBH
CSB-MP468972KBH
CSB-EP468972KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP468973KBH
CSB-EP468973KBH
CSB-BP468973KBH
CSB-MP468973KBH
CSB-EP468973KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Cobyric acid synthase (cobQ), partial CSB-YP468974KBH
CSB-EP468974KBH
CSB-BP468974KBH
CSB-MP468974KBH
CSB-EP468974KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP468975KBH
CSB-EP468975KBH
CSB-BP468975KBH
CSB-MP468975KBH
CSB-EP468975KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Sulfite reductase [NADPH] hemoprotein beta-component 1 (cysI1), partial CSB-YP468976KBH
CSB-EP468976KBH
CSB-BP468976KBH
CSB-MP468976KBH
CSB-EP468976KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA pseudouridine synthase D (truD) CSB-YP468977KBH
CSB-EP468977KBH
CSB-BP468977KBH
CSB-MP468977KBH
CSB-EP468977KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP468978KBH
CSB-EP468978KBH
CSB-BP468978KBH
CSB-MP468978KBH
CSB-EP468978KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein RnfH (rnfH) CSB-YP468979KBH
CSB-EP468979KBH
CSB-BP468979KBH
CSB-MP468979KBH
CSB-EP468979KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ribosome maturation factor RimM (rimM) CSB-YP468980KBH
CSB-EP468980KBH
CSB-BP468980KBH
CSB-MP468980KBH
CSB-EP468980KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP468981KBH
CSB-EP468981KBH
CSB-BP468981KBH
CSB-MP468981KBH
CSB-EP468981KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Chorismate synthase (aroC) CSB-YP468982KBH
CSB-EP468982KBH
CSB-BP468982KBH
CSB-MP468982KBH
CSB-EP468982KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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