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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP468943KBH
CSB-EP468943KBH
CSB-BP468943KBH
CSB-MP468943KBH
CSB-EP468943KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP468944KBH
CSB-EP468944KBH
CSB-BP468944KBH
CSB-MP468944KBH
CSB-EP468944KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Elongation factor P-like protein (KPK_1559) CSB-YP468945KBH
CSB-EP468945KBH
CSB-BP468945KBH
CSB-MP468945KBH
CSB-EP468945KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Adenine deaminase (ade), partial CSB-YP468946KBH
CSB-EP468946KBH
CSB-BP468946KBH
CSB-MP468946KBH
CSB-EP468946KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Multidrug resistance protein MdtB (mdtB), partial CSB-YP468947KBH
CSB-EP468947KBH
CSB-BP468947KBH
CSB-MP468947KBH
CSB-EP468947KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae DNA gyrase inhibitor (sbmC) CSB-YP468948KBH
CSB-EP468948KBH
CSB-BP468948KBH
CSB-MP468948KBH
CSB-EP468948KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Copper homeostasis protein CutC (cutC) CSB-YP468949KBH
CSB-EP468949KBH
CSB-BP468949KBH
CSB-MP468949KBH
CSB-EP468949KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP468950KBH
CSB-EP468950KBH
CSB-BP468950KBH
CSB-MP468950KBH
CSB-EP468950KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0059 membrane protein KPK_1956 (KPK_1956), partial CSB-YP468951KBH1
CSB-EP468951KBH1
CSB-BP468951KBH1
CSB-MP468951KBH1
CSB-EP468951KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Fatty acid metabolism regulator protein (fadR) CSB-YP468952KBH
CSB-EP468952KBH
CSB-BP468952KBH
CSB-MP468952KBH
CSB-EP468952KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Threonine--tRNA ligase (thrS), partial CSB-YP468953KBH
CSB-EP468953KBH
CSB-BP468953KBH
CSB-MP468953KBH
CSB-EP468953KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Trans-aconitate 2-methyltransferase (tam) CSB-YP468954KBH
CSB-EP468954KBH
CSB-BP468954KBH
CSB-MP468954KBH
CSB-EP468954KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae DNA replication terminus site-binding protein (tus) CSB-YP468955KBH
CSB-EP468955KBH
CSB-BP468955KBH
CSB-MP468955KBH
CSB-EP468955KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae GTP cyclohydrolase-2 (ribA) CSB-YP468956KBH
CSB-EP468956KBH
CSB-BP468956KBH
CSB-MP468956KBH
CSB-EP468956KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP468957KBH
CSB-EP468957KBH
CSB-BP468957KBH
CSB-MP468957KBH
CSB-EP468957KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP468958KBH
CSB-EP468958KBH
CSB-BP468958KBH
CSB-MP468958KBH
CSB-EP468958KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0306 protein KPK_0562 (KPK_0562) CSB-YP468959KBH
CSB-EP468959KBH
CSB-BP468959KBH
CSB-MP468959KBH
CSB-EP468959KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Tryptophan synthase alpha chain (trpA) CSB-YP468960KBH
CSB-EP468960KBH
CSB-BP468960KBH
CSB-MP468960KBH
CSB-EP468960KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae DNA replication and repair protein recF (recF) CSB-YP468961KBH
CSB-EP468961KBH
CSB-BP468961KBH
CSB-MP468961KBH
CSB-EP468961KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Small heat shock protein ibpB (ibpB) CSB-YP468962KBH
CSB-EP468962KBH
CSB-BP468962KBH
CSB-MP468962KBH
CSB-EP468962KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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