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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP469543DXN
CSB-EP469543DXN
CSB-BP469543DXN
CSB-MP469543DXN
CSB-EP469543DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP synthase gamma chain (atpG) CSB-YP469544DXN
CSB-EP469544DXN
CSB-BP469544DXN
CSB-MP469544DXN
CSB-EP469544DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Outer-membrane lipoprotein lolB (lolB) CSB-YP469545DXN
CSB-EP469545DXN
CSB-BP469545DXN
CSB-MP469545DXN
CSB-EP469545DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S20 (rpsT) CSB-YP469546DXN
CSB-EP469546DXN
CSB-BP469546DXN
CSB-MP469546DXN
CSB-EP469546DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L28 (rpmB) CSB-YP469547OED
CSB-EP469547OED
CSB-BP469547OED
CSB-MP469547OED
CSB-EP469547OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Protein translocase subunit SecA (secA), partial CSB-YP469548OED
CSB-EP469548OED
CSB-BP469548OED
CSB-MP469548OED
CSB-EP469548OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L19 (rplS) CSB-YP469549OED
CSB-EP469549OED
CSB-BP469549OED
CSB-MP469549OED
CSB-EP469549OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans DNA ligase (ligA), partial CSB-YP469550OED
CSB-EP469550OED
CSB-BP469550OED
CSB-MP469550OED
CSB-EP469550OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Ketol-acid reductoisomerase (ilvC) CSB-YP469551OED
CSB-EP469551OED
CSB-BP469551OED
CSB-MP469551OED
CSB-EP469551OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Pantothenate kinase (coaA) CSB-YP469552OED
CSB-EP469552OED
CSB-BP469552OED
CSB-MP469552OED
CSB-EP469552OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP469553OED
CSB-EP469553OED
CSB-BP469553OED
CSB-MP469553OED
CSB-EP469553OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Tryptophan synthase alpha chain (trpA) CSB-YP469554OED
CSB-EP469554OED
CSB-BP469554OED
CSB-MP469554OED
CSB-EP469554OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP469555OED
CSB-EP469555OED
CSB-BP469555OED
CSB-MP469555OED
CSB-EP469555OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans RNA pyrophosphohydrolase (rppH) CSB-YP469556OED
CSB-EP469556OED
CSB-BP469556OED
CSB-MP469556OED
CSB-EP469556OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP469557OED
CSB-EP469557OED
CSB-BP469557OED
CSB-MP469557OED
CSB-EP469557OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S12 (rpsL) CSB-YP469558OED
CSB-EP469558OED
CSB-BP469558OED
CSB-MP469558OED
CSB-EP469558OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L4 (rplD) CSB-YP469559OED
CSB-EP469559OED
CSB-BP469559OED
CSB-MP469559OED
CSB-EP469559OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP469560OED
CSB-EP469560OED
CSB-BP469560OED
CSB-MP469560OED
CSB-EP469560OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L18 (rplR) CSB-YP469561OED
CSB-EP469561OED
CSB-BP469561OED
CSB-MP469561OED
CSB-EP469561OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP469562OED
CSB-EP469562OED
CSB-BP469562OED
CSB-MP469562OED
CSB-EP469562OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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