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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi A tRNA (mo5U34)-methyltransferase CSB-YP468343SWT
CSB-EP468343SWT
CSB-BP468343SWT
CSB-MP468343SWT
CSB-EP468343SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0181 protein yoaH (yoaH) CSB-YP468344SWT
CSB-EP468344SWT
CSB-BP468344SWT
CSB-MP468344SWT
CSB-EP468344SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Ferrous iron transport protein C (feoC) CSB-YP468345SWT
CSB-EP468345SWT
CSB-BP468345SWT
CSB-MP468345SWT
CSB-EP468345SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Nickel-responsive regulator (nikR) CSB-YP468346SWT
CSB-EP468346SWT
CSB-BP468346SWT
CSB-MP468346SWT
CSB-EP468346SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 2,3-diketo-L-gulonate reductase (dlgD) CSB-YP468347SWT
CSB-EP468347SWT
CSB-BP468347SWT
CSB-MP468347SWT
CSB-EP468347SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP468348SWT
CSB-EP468348SWT
CSB-BP468348SWT
CSB-MP468348SWT
CSB-EP468348SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0758 protein yicR (yicR) CSB-YP468349SWT
CSB-EP468349SWT
CSB-BP468349SWT
CSB-MP468349SWT
CSB-EP468349SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP468350SWT
CSB-EP468350SWT
CSB-BP468350SWT
CSB-MP468350SWT
CSB-EP468350SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP468351SWT
CSB-EP468351SWT
CSB-BP468351SWT
CSB-MP468351SWT
CSB-EP468351SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Tryptophan synthase alpha chain (trpA) CSB-YP468352SWT
CSB-EP468352SWT
CSB-BP468352SWT
CSB-MP468352SWT
CSB-EP468352SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Putative transport protein YidE (yidE), partial CSB-YP468353SWT
CSB-EP468353SWT
CSB-BP468353SWT
CSB-MP468353SWT
CSB-EP468353SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Putative membrane protein insertion efficiency factor (yidD) CSB-YP468354SWT
CSB-EP468354SWT
CSB-BP468354SWT
CSB-MP468354SWT
CSB-EP468354SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A ATP synthase gamma chain (atpG) CSB-YP468355SWT
CSB-EP468355SWT
CSB-BP468355SWT
CSB-MP468355SWT
CSB-EP468355SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP468356SWT
CSB-EP468356SWT
CSB-BP468356SWT
CSB-MP468356SWT
CSB-EP468356SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A HTH-type transcriptional regulator hdfR (hdfR) CSB-YP468357SWT
CSB-EP468357SWT
CSB-BP468357SWT
CSB-MP468357SWT
CSB-EP468357SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Putative ECA polymerase (wzyE), partial CSB-YP468358SWT
CSB-EP468358SWT
CSB-BP468358SWT
CSB-MP468358SWT
CSB-EP468358SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A D-tyrosyl-tRNA (Tyr) deacylase CSB-YP468359SWT
CSB-EP468359SWT
CSB-BP468359SWT
CSB-MP468359SWT
CSB-EP468359SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A L-rhamnose isomerase (rhaA) CSB-YP468360SWT
CSB-EP468360SWT
CSB-BP468360SWT
CSB-MP468360SWT
CSB-EP468360SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A L-rhamnose-proton symporter (rhaT), partial CSB-YP468361SWT
CSB-EP468361SWT
CSB-BP468361SWT
CSB-MP468361SWT
CSB-EP468361SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Regulator of ribonuclease activity A (rraA) CSB-YP468362SWT
CSB-EP468362SWT
CSB-BP468362SWT
CSB-MP468362SWT
CSB-EP468362SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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