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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oligotropha carboxidovorans 5'-nucleotidase surE (surE) CSB-YP469563OED
CSB-EP469563OED
CSB-BP469563OED
CSB-MP469563OED
CSB-EP469563OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP469564OED
CSB-EP469564OED
CSB-BP469564OED
CSB-MP469564OED
CSB-EP469564OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Transcriptional repressor NrdR (nrdR) CSB-YP469565OED
CSB-EP469565OED
CSB-BP469565OED
CSB-MP469565OED
CSB-EP469565OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP469566OED
CSB-EP469566OED
CSB-BP469566OED
CSB-MP469566OED
CSB-EP469566OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP469567OED
CSB-EP469567OED
CSB-BP469567OED
CSB-MP469567OED
CSB-EP469567OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S2 (rpsB) CSB-YP469568OED
CSB-EP469568OED
CSB-BP469568OED
CSB-MP469568OED
CSB-EP469568OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Elongation factor P (efp) CSB-YP469569OED
CSB-EP469569OED
CSB-BP469569OED
CSB-MP469569OED
CSB-EP469569OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Phosphoglucosamine mutase (glmM) CSB-YP469570OED
CSB-EP469570OED
CSB-BP469570OED
CSB-MP469570OED
CSB-EP469570OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Probable transcriptional regulatory protein OCAR_7305/OCA5_c08120 (OCAR_7305, OCA5_c08120) CSB-YP469571OED
CSB-EP469571OED
CSB-BP469571OED
CSB-MP469571OED
CSB-EP469571OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L34 (rpmH) CSB-YP469572OED
CSB-EP469572OED
CSB-BP469572OED
CSB-MP469572OED
CSB-EP469572OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Adenylosuccinate synthetase (purA) CSB-YP469573HUY
CSB-EP469573HUY
CSB-BP469573HUY
CSB-MP469573HUY
CSB-EP469573HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L27 (rpmA) CSB-YP469574HUY
CSB-EP469574HUY
CSB-BP469574HUY
CSB-MP469574HUY
CSB-EP469574HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Putative Holliday junction resolvase (HPP12_0331) CSB-YP469575HUY
CSB-EP469575HUY
CSB-BP469575HUY
CSB-MP469575HUY
CSB-EP469575HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Dihydroorotate dehydrogenase (quinone) CSB-YP469576HUY
CSB-EP469576HUY
CSB-BP469576HUY
CSB-MP469576HUY
CSB-EP469576HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Glutamate racemase (murI) CSB-YP469577HUY
CSB-EP469577HUY
CSB-BP469577HUY
CSB-MP469577HUY
CSB-EP469577HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Dihydroorotase (pyrC) CSB-YP469578HUY
CSB-EP469578HUY
CSB-BP469578HUY
CSB-MP469578HUY
CSB-EP469578HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Glutamate--tRNA ligase 2 (gltX2) CSB-YP469579HUY
CSB-EP469579HUY
CSB-BP469579HUY
CSB-MP469579HUY
CSB-EP469579HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP469580HUY
CSB-EP469580HUY
CSB-BP469580HUY
CSB-MP469580HUY
CSB-EP469580HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Lipid-A-disaccharide synthase (lpxB) CSB-YP469581HUY
CSB-EP469581HUY
CSB-BP469581HUY
CSB-MP469581HUY
CSB-EP469581HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 5'-nucleotidase surE (surE) CSB-YP469582HUY
CSB-EP469582HUY
CSB-BP469582HUY
CSB-MP469582HUY
CSB-EP469582HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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