Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter baumannii 50S ribosomal protein L6 (rplF) CSB-YP472305AWM
CSB-EP472305AWM
CSB-BP472305AWM
CSB-MP472305AWM
CSB-EP472305AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 30S ribosomal protein S3 (rpsC) CSB-YP472306AWM
CSB-EP472306AWM
CSB-BP472306AWM
CSB-MP472306AWM
CSB-EP472306AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Shikimate dehydrogenase (aroE) CSB-YP472307AWM
CSB-EP472307AWM
CSB-BP472307AWM
CSB-MP472307AWM
CSB-EP472307AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 3-dehydroquinate synthase (aroB) CSB-YP472308AWM
CSB-EP472308AWM
CSB-BP472308AWM
CSB-MP472308AWM
CSB-EP472308AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Chromosomal replication initiator protein DnaA (dnaA) CSB-YP472309AWM
CSB-EP472309AWM
CSB-BP472309AWM
CSB-MP472309AWM
CSB-EP472309AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Chaperone protein DnaK (dnaK), partial CSB-YP472310AWM
CSB-EP472310AWM
CSB-BP472310AWM
CSB-MP472310AWM
CSB-EP472310AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP472311AWM
CSB-EP472311AWM
CSB-BP472311AWM
CSB-MP472311AWM
CSB-EP472311AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Phosphoglucosamine mutase (glmM) CSB-YP472312AWM
CSB-EP472312AWM
CSB-BP472312AWM
CSB-MP472312AWM
CSB-EP472312AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus UPF0133 protein THA_1374 (THA_1374) CSB-YP472313TNG
CSB-EP472313TNG
CSB-BP472313TNG
CSB-MP472313TNG
CSB-EP472313TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP472314TNG
CSB-EP472314TNG
CSB-BP472314TNG
CSB-MP472314TNG
CSB-EP472314TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Transcriptional repressor NrdR (nrdR) CSB-YP472315TNG
CSB-EP472315TNG
CSB-BP472315TNG
CSB-MP472315TNG
CSB-EP472315TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP472316TNG
CSB-EP472316TNG
CSB-BP472316TNG
CSB-MP472316TNG
CSB-EP472316TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP472317TNG
CSB-EP472317TNG
CSB-BP472317TNG
CSB-MP472317TNG
CSB-EP472317TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Ribosome-binding factor A (rbfA) CSB-YP472318TNG
CSB-EP472318TNG
CSB-BP472318TNG
CSB-MP472318TNG
CSB-EP472318TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Adenine deaminase (ade), partial CSB-YP472319TNG
CSB-EP472319TNG
CSB-BP472319TNG
CSB-MP472319TNG
CSB-EP472319TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Protein hfq (hfq) CSB-YP472320TNG
CSB-EP472320TNG
CSB-BP472320TNG
CSB-MP472320TNG
CSB-EP472320TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus UvrABC system protein B (uvrB), partial CSB-YP472321TNG
CSB-EP472321TNG
CSB-BP472321TNG
CSB-MP472321TNG
CSB-EP472321TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Thymidylate synthase thyX (thyX) CSB-YP472322TNG
CSB-EP472322TNG
CSB-BP472322TNG
CSB-MP472322TNG
CSB-EP472322TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 60 kDa chaperonin (groL), partial CSB-YP472323TNG
CSB-EP472323TNG
CSB-BP472323TNG
CSB-MP472323TNG
CSB-EP472323TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP472324TNG
CSB-EP472324TNG
CSB-BP472324TNG
CSB-MP472324TNG
CSB-EP472324TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>