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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coprothermobacter proteolyticus 30S ribosomal protein S3 (rpsC) CSB-YP473726DWR
CSB-EP473726DWR
CSB-BP473726DWR
CSB-MP473726DWR
CSB-EP473726DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP473727DWR
CSB-EP473727DWR
CSB-BP473727DWR
CSB-MP473727DWR
CSB-EP473727DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Thiamine-phosphate synthase (thiE) CSB-YP473728DKI
CSB-EP473728DKI
CSB-BP473728DKI
CSB-MP473728DKI
CSB-EP473728DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum UPF0133 protein DICTH_1981 (DICTH_1981) CSB-YP473729DKI
CSB-EP473729DKI
CSB-BP473729DKI
CSB-MP473729DKI
CSB-EP473729DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L25 (rplY) CSB-YP473730DKI
CSB-EP473730DKI
CSB-BP473730DKI
CSB-MP473730DKI
CSB-EP473730DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Glutamate 5-kinase (proB) CSB-YP473731DKI
CSB-EP473731DKI
CSB-BP473731DKI
CSB-MP473731DKI
CSB-EP473731DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Ribosomal protein L11 methyltransferase (prmA) CSB-YP473732DKI
CSB-EP473732DKI
CSB-BP473732DKI
CSB-MP473732DKI
CSB-EP473732DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L5 (rplE) CSB-YP473733DKI
CSB-EP473733DKI
CSB-BP473733DKI
CSB-MP473733DKI
CSB-EP473733DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L36 (rpmJ) CSB-YP473734DKI
CSB-EP473734DKI
CSB-BP473734DKI
CSB-MP473734DKI
CSB-EP473734DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S4 (rpsD) CSB-YP473735DKI
CSB-EP473735DKI
CSB-BP473735DKI
CSB-MP473735DKI
CSB-EP473735DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP473736DKI
CSB-EP473736DKI
CSB-BP473736DKI
CSB-MP473736DKI
CSB-EP473736DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum UPF0042 nucleotide-binding protein DICTH_1001 (DICTH_1001) CSB-YP473737DKI
CSB-EP473737DKI
CSB-BP473737DKI
CSB-MP473737DKI
CSB-EP473737DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP473738DKI
CSB-EP473738DKI
CSB-BP473738DKI
CSB-MP473738DKI
CSB-EP473738DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 2-isopropylmalate synthase (leuA), partial CSB-YP473739DKI
CSB-EP473739DKI
CSB-BP473739DKI
CSB-MP473739DKI
CSB-EP473739DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP473740DKI
CSB-EP473740DKI
CSB-BP473740DKI
CSB-MP473740DKI
CSB-EP473740DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L35 (rpmI) CSB-YP473741DKI
CSB-EP473741DKI
CSB-BP473741DKI
CSB-MP473741DKI
CSB-EP473741DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S18 (rpsR) CSB-YP473742DKI
CSB-EP473742DKI
CSB-BP473742DKI
CSB-MP473742DKI
CSB-EP473742DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L11 (rplK) CSB-YP473743DKI
CSB-EP473743DKI
CSB-BP473743DKI
CSB-MP473743DKI
CSB-EP473743DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Enolase (eno) CSB-YP473744DKI
CSB-EP473744DKI
CSB-BP473744DKI
CSB-MP473744DKI
CSB-EP473744DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum UvrABC system protein C (uvrC), partial CSB-YP473745DKI
CSB-EP473745DKI
CSB-BP473745DKI
CSB-MP473745DKI
CSB-EP473745DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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