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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aliivibrio salmonicida tRNA (uracil (54)-C (5))-methyltransferase CSB-YP474026AZM
CSB-EP474026AZM
CSB-BP474026AZM
CSB-MP474026AZM
CSB-EP474026AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 10 kDa chaperonin (groS) CSB-YP474027AZM
CSB-EP474027AZM
CSB-BP474027AZM
CSB-MP474027AZM
CSB-EP474027AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L34 (rpmH) CSB-YP474028AZM
CSB-EP474028AZM
CSB-BP474028AZM
CSB-MP474028AZM
CSB-EP474028AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S12 (rpsL) CSB-YP474029AZM
CSB-EP474029AZM
CSB-BP474029AZM
CSB-MP474029AZM
CSB-EP474029AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S17 (rpsQ) CSB-YP474030AZM
CSB-EP474030AZM
CSB-BP474030AZM
CSB-MP474030AZM
CSB-EP474030AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S11 (rpsK) CSB-YP474031AZM
CSB-EP474031AZM
CSB-BP474031AZM
CSB-MP474031AZM
CSB-EP474031AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida RNA polymerase-associated protein RapA (rapA), partial CSB-YP474032AZM
CSB-EP474032AZM
CSB-BP474032AZM
CSB-MP474032AZM
CSB-EP474032AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Glycogen synthase (glgA) CSB-YP474033AZM
CSB-EP474033AZM
CSB-BP474033AZM
CSB-MP474033AZM
CSB-EP474033AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida GMP reductase (guaC) CSB-YP474034AZM
CSB-EP474034AZM
CSB-BP474034AZM
CSB-MP474034AZM
CSB-EP474034AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Imidazolonepropionase (hutI) CSB-YP474035AZM
CSB-EP474035AZM
CSB-BP474035AZM
CSB-MP474035AZM
CSB-EP474035AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phosphonoacetaldehyde hydrolase (phnX) CSB-YP474036AZM
CSB-EP474036AZM
CSB-BP474036AZM
CSB-MP474036AZM
CSB-EP474036AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leishmania tarentolae Bifunctional helicase and thymine dioxygenase JBP2 (JBP2), partial CSB-YP474037LOI
CSB-EP474037LOI
CSB-BP474037LOI
CSB-MP474037LOI
CSB-EP474037LOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Protein sey1 (sey1), partial CSB-YP474038ETH
CSB-EP474038ETH
CSB-BP474038ETH
CSB-MP474038ETH
CSB-EP474038ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Probable cutinase 2 (Pc13g05110) CSB-YP474039ETH
CSB-EP474039ETH
CSB-BP474039ETH
CSB-MP474039ETH
CSB-EP474039ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Leucine aminopeptidase 1 (lap1) CSB-YP474040ETH
CSB-EP474040ETH
CSB-BP474040ETH
CSB-MP474040ETH
CSB-EP474040ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Catabolic 3-dehydroquinase (qutE) CSB-YP474041ETH
CSB-EP474041ETH
CSB-BP474041ETH
CSB-MP474041ETH
CSB-EP474041ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum U3 small nucleolar RNA-associated protein 25 (utp25), partial CSB-YP474042ETH
CSB-EP474042ETH
CSB-BP474042ETH
CSB-MP474042ETH
CSB-EP474042ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Ubiquinone biosynthesis protein coq4, mitochondrial (coq4) CSB-YP474043ETH
CSB-EP474043ETH
CSB-BP474043ETH
CSB-MP474043ETH
CSB-EP474043ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum ASTRA-associated protein 1 (asa1) CSB-YP474044ETH
CSB-EP474044ETH
CSB-BP474044ETH
CSB-MP474044ETH
CSB-EP474044ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Aspartic protease pep1 (pep1) CSB-YP474045ETH
CSB-EP474045ETH
CSB-BP474045ETH
CSB-MP474045ETH
CSB-EP474045ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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