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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelodictyon phaeoclathratiforme Proline--tRNA ligase (proS) CSB-YP474366ETB
CSB-EP474366ETB
CSB-BP474366ETB
CSB-MP474366ETB
CSB-EP474366ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme UPF0133 protein Ppha_1174 (Ppha_1174) CSB-YP474367ETB
CSB-EP474367ETB
CSB-BP474367ETB
CSB-MP474367ETB
CSB-EP474367ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Methionine--tRNA ligase (metG), partial CSB-YP474368ETB
CSB-EP474368ETB
CSB-BP474368ETB
CSB-MP474368ETB
CSB-EP474368ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP474369ETB1
CSB-EP474369ETB1
CSB-BP474369ETB1
CSB-MP474369ETB1
CSB-EP474369ETB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP474370ETB
CSB-EP474370ETB
CSB-BP474370ETB
CSB-MP474370ETB
CSB-EP474370ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP474371FLY
CSB-EP474371FLY
CSB-BP474371FLY
CSB-MP474371FLY
CSB-EP474371FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia HTH-type transcriptional regulator BetI (betI) CSB-YP474372FLY
CSB-EP474372FLY
CSB-BP474372FLY
CSB-MP474372FLY
CSB-EP474372FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 2-isopropylmalate synthase (leuA), partial CSB-YP474373FLY
CSB-EP474373FLY
CSB-BP474373FLY
CSB-MP474373FLY
CSB-EP474373FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP474374FLY
CSB-EP474374FLY
CSB-BP474374FLY
CSB-MP474374FLY
CSB-EP474374FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia NADH-quinone oxidoreductase subunit D 1 (nuoD1) CSB-YP474375FLY
CSB-EP474375FLY
CSB-BP474375FLY
CSB-MP474375FLY
CSB-EP474375FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glycerol kinase (glpK) CSB-YP474376FLY
CSB-EP474376FLY
CSB-BP474376FLY
CSB-MP474376FLY
CSB-EP474376FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP474377FLY
CSB-EP474377FLY
CSB-BP474377FLY
CSB-MP474377FLY
CSB-EP474377FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 60 kDa chaperonin (groL), partial CSB-YP474378FLY
CSB-BP474378FLY
CSB-MP474378FLY
CSB-EP474378FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glutamyl-tRNA reductase (hemA) CSB-YP474379FLY
CSB-EP474379FLY
CSB-BP474379FLY
CSB-MP474379FLY
CSB-EP474379FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S7 (rpsG) CSB-YP474380FLY
CSB-EP474380FLY
CSB-BP474380FLY
CSB-MP474380FLY
CSB-EP474380FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L6 (rplF) CSB-YP474381FLY
CSB-EP474381FLY
CSB-BP474381FLY
CSB-MP474381FLY
CSB-EP474381FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Anthranilate phosphoribosyltransferase (trpD) CSB-YP474382FLY
CSB-EP474382FLY
CSB-BP474382FLY
CSB-MP474382FLY
CSB-EP474382FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Peptidyl-Asp metalloendopeptidase (Smal_0815) CSB-YP474383FLY
CSB-EP474383FLY
CSB-BP474383FLY
CSB-MP474383FLY
CSB-EP474383FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia HPr kinase/phosphorylase (hprK) CSB-YP474384FLY
CSB-EP474384FLY
CSB-BP474384FLY
CSB-MP474384FLY
CSB-EP474384FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP474385FLY
CSB-EP474385FLY
CSB-BP474385FLY
CSB-MP474385FLY
CSB-EP474385FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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