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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP473766TMY
CSB-EP473766TMY
CSB-BP473766TMY
CSB-MP473766TMY
CSB-EP473766TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP473767TMY
CSB-EP473767TMY
CSB-BP473767TMY
CSB-MP473767TMY
CSB-EP473767TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Ketol-acid reductoisomerase (ilvC) CSB-YP473768TMY
CSB-EP473768TMY
CSB-BP473768TMY
CSB-MP473768TMY
CSB-EP473768TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Phosphopantetheine adenylyltransferase (coaD) CSB-YP473769TMY
CSB-EP473769TMY
CSB-BP473769TMY
CSB-MP473769TMY
CSB-EP473769TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S16 (rpsP) CSB-YP473770TMY
CSB-EP473770TMY
CSB-BP473770TMY
CSB-MP473770TMY
CSB-EP473770TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP473771TMY1
CSB-EP473771TMY1
CSB-BP473771TMY1
CSB-MP473771TMY1
CSB-EP473771TMY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Adenylosuccinate synthetase (purA) CSB-YP473772TMY
CSB-EP473772TMY
CSB-BP473772TMY
CSB-MP473772TMY
CSB-EP473772TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Proline--tRNA ligase (proS), partial CSB-YP473773TMY
CSB-EP473773TMY
CSB-BP473773TMY
CSB-MP473773TMY
CSB-EP473773TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP473774TMY
CSB-EP473774TMY
CSB-BP473774TMY
CSB-MP473774TMY
CSB-EP473774TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0061 protein ydiU (ydiU) CSB-YP473775EOE
CSB-EP473775EOE
CSB-BP473775EOE
CSB-MP473775EOE
CSB-EP473775EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L20 (rplT) CSB-YP473776EOE
CSB-EP473776EOE
CSB-BP473776EOE
CSB-MP473776EOE
CSB-EP473776EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 NH (3)-dependent NAD (+) synthetase CSB-YP473777EOE
CSB-EP473777EOE
CSB-BP473777EOE
CSB-MP473777EOE
CSB-EP473777EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP473778EOE
CSB-EP473778EOE
CSB-BP473778EOE
CSB-MP473778EOE
CSB-EP473778EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0267 protein yqfB (yqfB) CSB-YP473779EOE
CSB-EP473779EOE
CSB-BP473779EOE
CSB-MP473779EOE
CSB-EP473779EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cell division protein ZapA (zapA) CSB-YP473780EOE
CSB-EP473780EOE
CSB-BP473780EOE
CSB-MP473780EOE
CSB-EP473780EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 S-adenosylmethionine synthase (metK) CSB-YP473781EOE
CSB-EP473781EOE
CSB-BP473781EOE
CSB-MP473781EOE
CSB-EP473781EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP473782EOE
CSB-EP473782EOE
CSB-BP473782EOE
CSB-MP473782EOE
CSB-EP473782EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 LPS-assembly lipoprotein lptE (lptE) CSB-YP473783EOE
CSB-EP473783EOE
CSB-BP473783EOE
CSB-MP473783EOE
CSB-EP473783EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Potassium-transporting ATPase C chain (kdpC), partial CSB-YP473784EOE1
CSB-EP473784EOE1
CSB-BP473784EOE1
CSB-MP473784EOE1
CSB-EP473784EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP473785EOE
CSB-EP473785EOE
CSB-BP473785EOE
CSB-MP473785EOE
CSB-EP473785EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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