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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP474226DXN
CSB-EP474226DXN
CSB-BP474226DXN
CSB-MP474226DXN
CSB-EP474226DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP474227DXN
CSB-EP474227DXN
CSB-BP474227DXN
CSB-MP474227DXN
CSB-EP474227DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Bifunctional protein GlmU (glmU) CSB-YP474228DXN
CSB-EP474228DXN
CSB-BP474228DXN
CSB-MP474228DXN
CSB-EP474228DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Lipid-A-disaccharide synthase (lpxB) CSB-YP474229DXN
CSB-EP474229DXN
CSB-BP474229DXN
CSB-MP474229DXN
CSB-EP474229DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii GTPase obg (obg) CSB-YP474230DXN
CSB-EP474230DXN
CSB-BP474230DXN
CSB-MP474230DXN
CSB-EP474230DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Potassium-transporting ATPase C chain (kdpC), partial CSB-YP474231OED
CSB-EP474231OED
CSB-BP474231OED
CSB-MP474231OED
CSB-EP474231OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP474232OED
CSB-EP474232OED
CSB-BP474232OED
CSB-MP474232OED
CSB-EP474232OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Diaminopimelate epimerase (dapF) CSB-YP474233OED
CSB-EP474233OED
CSB-BP474233OED
CSB-MP474233OED
CSB-EP474233OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP474234OED
CSB-EP474234OED
CSB-BP474234OED
CSB-MP474234OED
CSB-EP474234OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Flagellar L-ring protein (flgH) CSB-YP474235OED
CSB-EP474235OED
CSB-BP474235OED
CSB-MP474235OED
CSB-EP474235OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans UPF0335 protein OCAR_5086/OCA5_c28780 (OCAR_5086, OCA5_c28780) CSB-YP474236OED
CSB-EP474236OED
CSB-BP474236OED
CSB-MP474236OED
CSB-EP474236OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP474237OED
CSB-EP474237OED
CSB-BP474237OED
CSB-MP474237OED
CSB-EP474237OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L20 (rplT) CSB-YP474238OED
CSB-EP474238OED
CSB-BP474238OED
CSB-MP474238OED
CSB-EP474238OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP474239OED
CSB-EP474239OED
CSB-BP474239OED
CSB-MP474239OED
CSB-EP474239OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Ribosome-binding factor A (rbfA) CSB-YP474240OED
CSB-EP474240OED
CSB-BP474240OED
CSB-MP474240OED
CSB-EP474240OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Protein ApaG (apaG) CSB-YP474241OED
CSB-EP474241OED
CSB-BP474241OED
CSB-MP474241OED
CSB-EP474241OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S4 (rpsD) CSB-YP474242OED
CSB-EP474242OED
CSB-BP474242OED
CSB-MP474242OED
CSB-EP474242OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L11 (rplK) CSB-YP474243OED
CSB-EP474243OED
CSB-BP474243OED
CSB-MP474243OED
CSB-EP474243OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S3 (rpsC) CSB-YP474244OED
CSB-EP474244OED
CSB-BP474244OED
CSB-MP474244OED
CSB-EP474244OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L5 (rplE) CSB-YP474245OED
CSB-EP474245OED
CSB-BP474245OED
CSB-MP474245OED
CSB-EP474245OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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