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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella newport Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP474426SWS
CSB-EP474426SWS
CSB-BP474426SWS
CSB-MP474426SWS
CSB-EP474426SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport DNA ligase B (ligB), partial CSB-YP474427SWS
CSB-EP474427SWS
CSB-BP474427SWS
CSB-MP474427SWS
CSB-EP474427SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP474428SWS
CSB-EP474428SWS
CSB-BP474428SWS
CSB-MP474428SWS
CSB-EP474428SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Probable lipid kinase YegS (yegS) CSB-YP474429SWS
CSB-EP474429SWS
CSB-BP474429SWS
CSB-MP474429SWS
CSB-EP474429SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Probable endonuclease 4 (nfo) CSB-YP474430SWS
CSB-EP474430SWS
CSB-BP474430SWS
CSB-MP474430SWS
CSB-EP474430SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport D-galactonate dehydratase (dgoD) CSB-YP474431SWS
CSB-EP474431SWS
CSB-BP474431SWS
CSB-MP474431SWS
CSB-EP474431SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ATP synthase gamma chain (atpG) CSB-YP474432SWS
CSB-EP474432SWS
CSB-BP474432SWS
CSB-MP474432SWS
CSB-EP474432SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ATPase ravA (ravA) CSB-YP474433SWS
CSB-EP474433SWS
CSB-BP474433SWS
CSB-MP474433SWS
CSB-EP474433SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Protein CrcB homolog (crcB), partial CSB-YP474434SWS1
CSB-EP474434SWS1
CSB-BP474434SWS1
CSB-MP474434SWS1
CSB-EP474434SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Nucleoid-associated protein YejK (yejK) CSB-YP474435SWS
CSB-EP474435SWS
CSB-BP474435SWS
CSB-MP474435SWS
CSB-EP474435SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) CSB-YP474436SWS
CSB-EP474436SWS
CSB-BP474436SWS
CSB-MP474436SWS
CSB-EP474436SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Lipopolysaccharide core heptose (II)-phosphate phosphatase CSB-YP474437SWS
CSB-EP474437SWS
CSB-BP474437SWS
CSB-MP474437SWS
CSB-EP474437SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP474438SWS
CSB-EP474438SWS
CSB-BP474438SWS
CSB-MP474438SWS
CSB-EP474438SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0208 membrane protein YfbV (yfbV) CSB-YP474439SWS
CSB-EP474439SWS
CSB-BP474439SWS
CSB-MP474439SWS
CSB-EP474439SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Probable ubiquinone biosynthesis protein UbiB (ubiB) CSB-YP474440SWS
CSB-EP474440SWS
CSB-BP474440SWS
CSB-MP474440SWS
CSB-EP474440SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Potassium-transporting ATPase C chain (kdpC), partial CSB-YP474441SWS1
CSB-EP474441SWS1
CSB-BP474441SWS1
CSB-MP474441SWS1
CSB-EP474441SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP474442SWS
CSB-EP474442SWS
CSB-BP474442SWS
CSB-MP474442SWS
CSB-EP474442SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP474443SWS
CSB-EP474443SWS
CSB-BP474443SWS
CSB-MP474443SWS
CSB-EP474443SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0761 membrane protein yihY (yihY) CSB-YP474444SWS
CSB-EP474444SWS
CSB-BP474444SWS
CSB-MP474444SWS
CSB-EP474444SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport L-rhamnose-proton symporter (rhaT), partial CSB-YP474445SWS
CSB-EP474445SWS
CSB-BP474445SWS
CSB-MP474445SWS
CSB-EP474445SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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