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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli 30S ribosomal protein S14 (rpsN) CSB-YP475826ENW
CSB-EP475826ENW
CSB-BP475826ENW
CSB-MP475826ENW
CSB-EP475826ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L29 (rpmC) CSB-YP475827ENW
CSB-EP475827ENW
CSB-BP475827ENW
CSB-MP475827ENW
CSB-EP475827ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S7 (rpsG) CSB-YP475828ENW
CSB-EP475828ENW
CSB-BP475828ENW
CSB-MP475828ENW
CSB-EP475828ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fumarate reductase subunit D (frdD), partial CSB-YP475829ENW1
CSB-EP475829ENW1
CSB-BP475829ENW1
CSB-MP475829ENW1
CSB-EP475829ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (ulaD) CSB-YP475830ENW
CSB-EP475830ENW
CSB-BP475830ENW
CSB-MP475830ENW
CSB-EP475830ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP475831ENW
CSB-EP475831ENW
CSB-BP475831ENW
CSB-MP475831ENW
CSB-EP475831ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate carbamoyltransferase (pyrB) CSB-YP475832ENW
CSB-EP475832ENW
CSB-BP475832ENW
CSB-MP475832ENW
CSB-EP475832ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0442 protein yjjB (yjjB), partial CSB-YP475833ENW1
CSB-EP475833ENW1
CSB-BP475833ENW1
CSB-MP475833ENW1
CSB-EP475833ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Siroheme synthase (cysG) CSB-YP475834ENW
CSB-EP475834ENW
CSB-BP475834ENW
CSB-MP475834ENW
CSB-EP475834ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thiosulfate sulfurtransferase glpE (glpE) CSB-YP475835ENW
CSB-EP475835ENW
CSB-BP475835ENW
CSB-MP475835ENW
CSB-EP475835ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP475836ENW
CSB-EP475836ENW
CSB-BP475836ENW
CSB-MP475836ENW
CSB-EP475836ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-seryl-tRNA (Sec) selenium transferase CSB-YP475837ENW
CSB-EP475837ENW
CSB-BP475837ENW
CSB-MP475837ENW
CSB-EP475837ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphopantetheine adenylyltransferase (coaD) CSB-YP475838ENW
CSB-EP475838ENW
CSB-BP475838ENW
CSB-MP475838ENW
CSB-EP475838ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nucleoid occlusion factor SlmA (slmA) CSB-YP475839ENW
CSB-EP475839ENW
CSB-BP475839ENW
CSB-MP475839ENW
CSB-EP475839ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Small heat shock protein ibpA (ibpA) CSB-YP475840ENW
CSB-EP475840ENW
CSB-BP475840ENW
CSB-MP475840ENW
CSB-EP475840ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA modification GTPase MnmE (mnmE) CSB-YP475841ENW
CSB-EP475841ENW
CSB-BP475841ENW
CSB-MP475841ENW
CSB-EP475841ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase gamma chain (atpG) CSB-YP475842ENW
CSB-EP475842ENW
CSB-BP475842ENW
CSB-MP475842ENW
CSB-EP475842ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate--ammonia ligase (asnA) CSB-YP475843ENW
CSB-EP475843ENW
CSB-BP475843ENW
CSB-MP475843ENW
CSB-EP475843ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multifunctional CCA protein (cca) CSB-YP475844ENW
CSB-EP475844ENW
CSB-BP475844ENW
CSB-MP475844ENW
CSB-EP475844ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0597 protein yhaM (yhaM) CSB-YP475845ENW
CSB-EP475845ENW
CSB-BP475845ENW
CSB-MP475845ENW
CSB-EP475845ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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