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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Vibrio fischeri Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP475026VFC
CSB-EP475026VFC
CSB-BP475026VFC
CSB-MP475026VFC
CSB-EP475026VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP475027VFC
CSB-EP475027VFC
CSB-BP475027VFC
CSB-MP475027VFC
CSB-EP475027VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP475028VFC
CSB-EP475028VFC
CSB-BP475028VFC
CSB-MP475028VFC
CSB-EP475028VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP475029VFC
CSB-EP475029VFC
CSB-BP475029VFC
CSB-MP475029VFC
CSB-EP475029VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 30S ribosomal protein S20 (rpsT) CSB-YP475030SWN
CSB-EP475030SWN
CSB-BP475030SWN
CSB-MP475030SWN
CSB-EP475030SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP475031SWN
CSB-EP475031SWN
CSB-BP475031SWN
CSB-MP475031SWN
CSB-EP475031SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin G/U mismatch-specific DNA glycosylase (mug) CSB-YP475032SWN
CSB-EP475032SWN
CSB-BP475032SWN
CSB-MP475032SWN
CSB-EP475032SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Threonine/serine transporter TdcC (tdcC), partial CSB-YP475033SWN
CSB-EP475033SWN
CSB-BP475033SWN
CSB-MP475033SWN
CSB-EP475033SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 2-isopropylmalate synthase (leuA), partial CSB-YP475034SWN
CSB-EP475034SWN
CSB-BP475034SWN
CSB-MP475034SWN
CSB-EP475034SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP475035SWN
CSB-EP475035SWN
CSB-BP475035SWN
CSB-MP475035SWN
CSB-EP475035SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Electron transport complex protein RnfB (rnfB) CSB-YP475036SWN
CSB-EP475036SWN
CSB-BP475036SWN
CSB-MP475036SWN
CSB-EP475036SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin HTH-type transcriptional repressor PurR (purR) CSB-YP475037SWN
CSB-EP475037SWN
CSB-BP475037SWN
CSB-MP475037SWN
CSB-EP475037SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP475038SWN1
CSB-EP475038SWN1
CSB-BP475038SWN1
CSB-MP475038SWN1
CSB-EP475038SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB), partial CSB-YP475039SWN
CSB-EP475039SWN
CSB-BP475039SWN
CSB-MP475039SWN
CSB-EP475039SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP475040SWN
CSB-EP475040SWN
CSB-BP475040SWN
CSB-MP475040SWN
CSB-EP475040SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 30S ribosomal protein S2 (rpsB) CSB-YP475041SWN
CSB-EP475041SWN
CSB-BP475041SWN
CSB-MP475041SWN
CSB-EP475041SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP475042SWN
CSB-EP475042SWN
CSB-BP475042SWN
CSB-MP475042SWN
CSB-EP475042SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Quinate/shikimate dehydrogenase (ydiB) CSB-YP475043SWN
CSB-EP475043SWN
CSB-BP475043SWN
CSB-MP475043SWN
CSB-EP475043SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L35 (rpmI) CSB-YP475044SWN
CSB-EP475044SWN
CSB-BP475044SWN
CSB-MP475044SWN
CSB-EP475044SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Arginine N-succinyltransferase (astA) CSB-YP475045SWN
CSB-EP475045SWN
CSB-BP475045SWN
CSB-MP475045SWN
CSB-EP475045SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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