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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli UPF0257 lipoprotein ynfC (ynfC) CSB-YP475886ENW
CSB-EP475886ENW
CSB-BP475886ENW
CSB-MP475886ENW
CSB-EP475886ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Electron transport complex protein RnfB (rnfB) CSB-YP475887ENW
CSB-EP475887ENW
CSB-BP475887ENW
CSB-MP475887ENW
CSB-EP475887ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional repressor PurR (purR) CSB-YP475888ENW
CSB-EP475888ENW
CSB-BP475888ENW
CSB-MP475888ENW
CSB-EP475888ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Threonine--tRNA ligase (thrS) CSB-YP475889ENW
CSB-EP475889ENW
CSB-BP475889ENW
CSB-MP475889ENW
CSB-EP475889ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP475890ENW
CSB-EP475890ENW
CSB-BP475890ENW
CSB-MP475890ENW
CSB-EP475890ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP475891ENW
CSB-EP475891ENW
CSB-BP475891ENW
CSB-MP475891ENW
CSB-EP475891ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP475892RLY
CSB-EP475892RLY
CSB-BP475892RLY
CSB-MP475892RLY
CSB-EP475892RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP475893RLY
CSB-EP475893RLY
CSB-BP475893RLY
CSB-MP475893RLY
CSB-EP475893RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Aspartate--tRNA ligase (aspS) CSB-YP475894RLY
CSB-EP475894RLY
CSB-BP475894RLY
CSB-MP475894RLY
CSB-EP475894RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP475895RLY
CSB-EP475895RLY
CSB-BP475895RLY
CSB-MP475895RLY
CSB-EP475895RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Probable transcriptional regulatory protein RC1_1808 (RC1_1808) CSB-YP475896RLY
CSB-EP475896RLY
CSB-BP475896RLY
CSB-MP475896RLY
CSB-EP475896RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UPF0178 protein RC1_2062 (RC1_2062) CSB-YP475897RLY
CSB-EP475897RLY
CSB-BP475897RLY
CSB-MP475897RLY
CSB-EP475897RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum ATP synthase epsilon chain (atpC) CSB-YP475898RLY
CSB-EP475898RLY
CSB-BP475898RLY
CSB-MP475898RLY
CSB-EP475898RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP475899RLY
CSB-EP475899RLY
CSB-BP475899RLY
CSB-MP475899RLY
CSB-EP475899RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Protein CrcB homolog (crcB), partial CSB-YP475900RLY1
CSB-EP475900RLY1
CSB-BP475900RLY1
CSB-MP475900RLY1
CSB-EP475900RLY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP475901RLY
CSB-EP475901RLY
CSB-BP475901RLY
CSB-MP475901RLY
CSB-EP475901RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 50S ribosomal protein L10 (rplJ) CSB-YP475902RLY
CSB-EP475902RLY
CSB-BP475902RLY
CSB-MP475902RLY
CSB-EP475902RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP475903RLY
CSB-EP475903RLY
CSB-BP475903RLY
CSB-MP475903RLY
CSB-EP475903RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 50S ribosomal protein L4 (rplD) CSB-YP475904RLY
CSB-EP475904RLY
CSB-BP475904RLY
CSB-MP475904RLY
CSB-EP475904RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP475905RLY
CSB-EP475905RLY
CSB-BP475905RLY
CSB-MP475905RLY
CSB-EP475905RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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