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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S7 (rpsG) CSB-YP475486DKI
CSB-EP475486DKI
CSB-BP475486DKI
CSB-MP475486DKI
CSB-EP475486DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S14 type Z (rpsZ) CSB-YP475487DKI
CSB-EP475487DKI
CSB-BP475487DKI
CSB-MP475487DKI
CSB-EP475487DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L18 (rplR) CSB-YP475488DKI
CSB-EP475488DKI
CSB-BP475488DKI
CSB-MP475488DKI
CSB-EP475488DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Shikimate dehydrogenase (aroE) CSB-YP475489DKI
CSB-EP475489DKI
CSB-BP475489DKI
CSB-MP475489DKI
CSB-EP475489DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum DNA mismatch repair protein mutL (mutL), partial CSB-YP475490DKI
CSB-EP475490DKI
CSB-BP475490DKI
CSB-MP475490DKI
CSB-EP475490DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP475491DKI
CSB-EP475491DKI
CSB-BP475491DKI
CSB-MP475491DKI
CSB-EP475491DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Phosphopantetheine adenylyltransferase (coaD) CSB-YP475492DKI
CSB-EP475492DKI
CSB-BP475492DKI
CSB-MP475492DKI
CSB-EP475492DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP475493DKI
CSB-EP475493DKI
CSB-BP475493DKI
CSB-MP475493DKI
CSB-EP475493DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP475494DKI
CSB-EP475494DKI
CSB-BP475494DKI
CSB-MP475494DKI
CSB-EP475494DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Gamma-glutamyl phosphate reductase (proA) CSB-YP475495DKI
CSB-EP475495DKI
CSB-BP475495DKI
CSB-MP475495DKI
CSB-EP475495DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Ribonuclease PH (rph) CSB-YP475496DKI
CSB-EP475496DKI
CSB-BP475496DKI
CSB-MP475496DKI
CSB-EP475496DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L10 (rplJ) CSB-YP475497DKI
CSB-EP475497DKI
CSB-BP475497DKI
CSB-MP475497DKI
CSB-EP475497DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP475498DKI
CSB-EP475498DKI
CSB-BP475498DKI
CSB-MP475498DKI
CSB-EP475498DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP475499DKI
CSB-EP475499DKI
CSB-BP475499DKI
CSB-MP475499DKI
CSB-EP475499DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum UPF0272 protein DICTH_1434 (DICTH_1434) CSB-YP475500DKI
CSB-EP475500DKI
CSB-BP475500DKI
CSB-MP475500DKI
CSB-EP475500DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP475501DKI
CSB-EP475501DKI
CSB-BP475501DKI
CSB-MP475501DKI
CSB-EP475501DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP475502TMY
CSB-EP475502TMY
CSB-BP475502TMY
CSB-MP475502TMY
CSB-EP475502TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L2 (rplB) CSB-YP475503TMY
CSB-EP475503TMY
CSB-BP475503TMY
CSB-MP475503TMY
CSB-EP475503TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S7 (rpsG) CSB-YP475504TMY
CSB-EP475504TMY
CSB-BP475504TMY
CSB-MP475504TMY
CSB-EP475504TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Chaperone protein DnaK (dnaK), partial CSB-YP475505TMY
CSB-EP475505TMY
CSB-BP475505TMY
CSB-MP475505TMY
CSB-EP475505TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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