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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermococcus onnurineus Glycerol kinase (glpK) CSB-YP476086TMR
CSB-EP476086TMR
CSB-BP476086TMR
CSB-MP476086TMR
CSB-EP476086TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S27e (rps27e) CSB-YP476087TMR
CSB-EP476087TMR
CSB-BP476087TMR
CSB-MP476087TMR
CSB-EP476087TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Uracil phosphoribosyltransferase (upp) CSB-YP476088TMR
CSB-EP476088TMR
CSB-BP476088TMR
CSB-MP476088TMR
CSB-EP476088TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP476089TMR
CSB-EP476089TMR
CSB-BP476089TMR
CSB-MP476089TMR
CSB-EP476089TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Thymidylate synthase thyX (thyX) CSB-YP476090TMR
CSB-EP476090TMR
CSB-BP476090TMR
CSB-MP476090TMR
CSB-EP476090TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Uncharacterized DAPA-like lyase TON_0506 (dapAL) CSB-YP476091TMR
CSB-EP476091TMR
CSB-BP476091TMR
CSB-MP476091TMR
CSB-EP476091TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0290 protein TON_1624 (TON_1624), partial CSB-YP476092TMR1
CSB-EP476092TMR1
CSB-BP476092TMR1
CSB-MP476092TMR1
CSB-EP476092TMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP476093TMR
CSB-EP476093TMR
CSB-BP476093TMR
CSB-MP476093TMR
CSB-EP476093TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP476094TMR
CSB-EP476094TMR
CSB-BP476094TMR
CSB-MP476094TMR
CSB-EP476094TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP476095TMR
CSB-EP476095TMR
CSB-BP476095TMR
CSB-MP476095TMR
CSB-EP476095TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus V-type ATP synthase subunit F (atpF) CSB-YP476096TMR
CSB-EP476096TMR
CSB-BP476096TMR
CSB-MP476096TMR
CSB-EP476096TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Cobalamin synthase (cobS), partial CSB-YP476097TMR1
CSB-EP476097TMR1
CSB-BP476097TMR1
CSB-MP476097TMR1
CSB-EP476097TMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S10P (rps10p) CSB-YP476098TMR
CSB-EP476098TMR
CSB-BP476098TMR
CSB-MP476098TMR
CSB-EP476098TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP476099TMR
CSB-EP476099TMR
CSB-BP476099TMR
CSB-MP476099TMR
CSB-EP476099TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S24e (rps24e) CSB-YP476100TMR
CSB-EP476100TMR
CSB-BP476100TMR
CSB-MP476100TMR
CSB-EP476100TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus ATP phosphoribosyltransferase (hisG) CSB-YP476101TMR
CSB-EP476101TMR
CSB-BP476101TMR
CSB-MP476101TMR
CSB-EP476101TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0248 protein TON_0940 (TON_0940) CSB-YP476102TMR
CSB-EP476102TMR
CSB-BP476102TMR
CSB-MP476102TMR
CSB-EP476102TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Tyrosine--tRNA ligase (tyrS) CSB-YP476103TMR
CSB-EP476103TMR
CSB-BP476103TMR
CSB-MP476103TMR
CSB-EP476103TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Maf-like protein TON_1078 (TON_1078) CSB-YP476104TMR
CSB-EP476104TMR
CSB-BP476104TMR
CSB-MP476104TMR
CSB-EP476104TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L39e (rpl39e) CSB-YP476105TMR
CSB-EP476105TMR
CSB-BP476105TMR
CSB-MP476105TMR
CSB-EP476105TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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