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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae Cytoskeleton protein rodZ (rodZ), partial CSB-YP475386KBH
CSB-EP475386KBH
CSB-BP475386KBH
CSB-MP475386KBH
CSB-EP475386KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP475387KBH
CSB-EP475387KBH
CSB-BP475387KBH
CSB-MP475387KBH
CSB-EP475387KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP475388KBH
CSB-EP475388KBH
CSB-BP475388KBH
CSB-MP475388KBH
CSB-EP475388KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP475389KBH
CSB-EP475389KBH
CSB-BP475389KBH
CSB-MP475389KBH
CSB-EP475389KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L25 (rplY) CSB-YP475390KBH
CSB-EP475390KBH
CSB-BP475390KBH
CSB-MP475390KBH
CSB-EP475390KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Methionine--tRNA ligase (metG), partial CSB-YP475391KBH
CSB-EP475391KBH
CSB-BP475391KBH
CSB-MP475391KBH
CSB-EP475391KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP475392KBH
CSB-EP475392KBH
CSB-BP475392KBH
CSB-MP475392KBH
CSB-EP475392KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Nitrogenase iron protein (nifH) CSB-YP475393KBH
CSB-EP475393KBH
CSB-BP475393KBH
CSB-MP475393KBH
CSB-EP475393KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein mtfA (mtfA) CSB-YP475394KBH
CSB-EP475394KBH
CSB-BP475394KBH
CSB-MP475394KBH
CSB-EP475394KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Arginine--tRNA ligase (argS), partial CSB-YP475395KBH
CSB-EP475395KBH
CSB-BP475395KBH
CSB-MP475395KBH
CSB-EP475395KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP475396KBH
CSB-EP475396KBH
CSB-BP475396KBH
CSB-MP475396KBH
CSB-EP475396KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein mgrB homolog (mgrB), partial CSB-YP475397KBH
CSB-EP475397KBH
CSB-BP475397KBH
CSB-MP475397KBH
CSB-EP475397KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Probable septum site-determining protein MinC (minC) CSB-YP475398KBH
CSB-EP475398KBH
CSB-BP475398KBH
CSB-MP475398KBH
CSB-EP475398KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP475399KBH
CSB-EP475399KBH
CSB-BP475399KBH
CSB-MP475399KBH
CSB-EP475399KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L20 (rplT) CSB-YP475400KBH
CSB-EP475400KBH
CSB-BP475400KBH
CSB-MP475400KBH
CSB-EP475400KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, partial CSB-YP475401KBH
CSB-EP475401KBH
CSB-BP475401KBH
CSB-MP475401KBH
CSB-EP475401KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphoribosyl-dephospho-CoA transferase (mdcG) CSB-YP475402KBH
CSB-EP475402KBH
CSB-BP475402KBH
CSB-MP475402KBH
CSB-EP475402KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0229 protein KPK_3252 (KPK_3252) CSB-YP475403KBH
CSB-EP475403KBH
CSB-BP475403KBH
CSB-MP475403KBH
CSB-EP475403KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Peptidase T (pepT) CSB-YP475404KBH
CSB-EP475404KBH
CSB-BP475404KBH
CSB-MP475404KBH
CSB-EP475404KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L13 (rplM) CSB-YP475405KBH
CSB-EP475405KBH
CSB-BP475405KBH
CSB-MP475405KBH
CSB-EP475405KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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