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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio fischeri 30S ribosomal protein S16 (rpsP) CSB-YP477326VFC
CSB-EP477326VFC
CSB-BP477326VFC
CSB-MP477326VFC
CSB-EP477326VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Enolase (eno) CSB-YP477327VFC
CSB-EP477327VFC
CSB-BP477327VFC
CSB-MP477327VFC
CSB-EP477327VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0246 protein VFMJ11_2214 (VFMJ11_2214) CSB-YP477328VFC
CSB-EP477328VFC
CSB-BP477328VFC
CSB-MP477328VFC
CSB-EP477328VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP477329VFC
CSB-EP477329VFC
CSB-BP477329VFC
CSB-MP477329VFC
CSB-EP477329VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Co-chaperone protein hscB homolog (hscB) CSB-YP477330VFC
CSB-EP477330VFC
CSB-BP477330VFC
CSB-MP477330VFC
CSB-EP477330VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP477331VFC
CSB-EP477331VFC
CSB-BP477331VFC
CSB-MP477331VFC
CSB-EP477331VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Bifunctional protein HldE (hldE) CSB-YP477332VFC
CSB-EP477332VFC
CSB-BP477332VFC
CSB-MP477332VFC
CSB-EP477332VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosomal protein S6 modification protein (rimK) CSB-YP477333VFC
CSB-EP477333VFC
CSB-BP477333VFC
CSB-MP477333VFC
CSB-EP477333VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Urease subunit beta (ureB) CSB-YP477334VFC
CSB-EP477334VFC
CSB-BP477334VFC
CSB-MP477334VFC
CSB-EP477334VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri tRNA sulfurtransferase (thiI) CSB-YP477335VFC
CSB-EP477335VFC
CSB-BP477335VFC
CSB-MP477335VFC
CSB-EP477335VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 3-dehydroquinate synthase (aroB) CSB-YP477336VFC
CSB-EP477336VFC
CSB-BP477336VFC
CSB-MP477336VFC
CSB-EP477336VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L9 (rplI) CSB-YP477337VFC
CSB-EP477337VFC
CSB-BP477337VFC
CSB-MP477337VFC
CSB-EP477337VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Glutamyl-tRNA reductase (hemA) CSB-YP477338VFC
CSB-EP477338VFC
CSB-BP477338VFC
CSB-MP477338VFC
CSB-EP477338VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 3-dehydroquinate dehydratase (aroQ) CSB-YP477339VFC
CSB-EP477339VFC
CSB-BP477339VFC
CSB-MP477339VFC
CSB-EP477339VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L10 (rplJ) CSB-YP477340VFC
CSB-EP477340VFC
CSB-BP477340VFC
CSB-MP477340VFC
CSB-EP477340VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri tRNA (uracil (54)-C (5))-methyltransferase CSB-YP477341VFC
CSB-EP477341VFC
CSB-BP477341VFC
CSB-MP477341VFC
CSB-EP477341VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Putative reductase VFMJ11_0926 (VFMJ11_0926) CSB-YP477342VFC
CSB-EP477342VFC
CSB-BP477342VFC
CSB-MP477342VFC
CSB-EP477342VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Aspartate--tRNA ligase (aspS), partial CSB-YP477343VFC
CSB-EP477343VFC
CSB-BP477343VFC
CSB-MP477343VFC
CSB-EP477343VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Protein smg homolog (smg) CSB-YP477344VFC
CSB-EP477344VFC
CSB-BP477344VFC
CSB-MP477344VFC
CSB-EP477344VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Dihydroxy-acid dehydratase (ilvD), partial CSB-YP477345VFC
CSB-EP477345VFC
CSB-BP477345VFC
CSB-MP477345VFC
CSB-EP477345VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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