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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae 50S ribosomal protein L9 (rplI) CSB-YP475466KBH
CSB-EP475466KBH
CSB-BP475466KBH
CSB-MP475466KBH
CSB-EP475466KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae DNA mismatch repair protein mutL (mutL), partial CSB-YP475467KBH
CSB-EP475467KBH
CSB-BP475467KBH
CSB-MP475467KBH
CSB-EP475467KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 10 kDa chaperonin (groS) CSB-YP475468KBH
CSB-EP475468KBH
CSB-BP475468KBH
CSB-MP475468KBH
CSB-EP475468KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Hydroxyethylthiazole kinase (thiM) CSB-YP475469DWR
CSB-EP475469DWR
CSB-BP475469DWR
CSB-MP475469DWR
CSB-EP475469DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 50S ribosomal protein L28 (rpmB) CSB-YP475470DWR
CSB-EP475470DWR
CSB-BP475470DWR
CSB-MP475470DWR
CSB-EP475470DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus GTPase obg (obg) CSB-YP475471DWR
CSB-EP475471DWR
CSB-BP475471DWR
CSB-MP475471DWR
CSB-EP475471DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Pantothenate synthetase (panC) CSB-YP475472DWR
CSB-EP475472DWR
CSB-BP475472DWR
CSB-MP475472DWR
CSB-EP475472DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus V-type ATP synthase subunit D (atpD) CSB-YP475473DWR
CSB-EP475473DWR
CSB-BP475473DWR
CSB-MP475473DWR
CSB-EP475473DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 50S ribosomal protein L19 (rplS) CSB-YP475474DWR
CSB-EP475474DWR
CSB-BP475474DWR
CSB-MP475474DWR
CSB-EP475474DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP475475DWR
CSB-EP475475DWR
CSB-BP475475DWR
CSB-MP475475DWR
CSB-EP475475DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Proline--tRNA ligase (proS), partial CSB-YP475476DWR
CSB-EP475476DWR
CSB-BP475476DWR
CSB-MP475476DWR
CSB-EP475476DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Probable butyrate kinase (buk) CSB-YP475477DWR
CSB-EP475477DWR
CSB-BP475477DWR
CSB-MP475477DWR
CSB-EP475477DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Ribosome-binding factor A (rbfA) CSB-YP475478DWR
CSB-EP475478DWR
CSB-BP475478DWR
CSB-MP475478DWR
CSB-EP475478DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus 50S ribosomal protein L29 (rpmC) CSB-YP475479DWR
CSB-EP475479DWR
CSB-BP475479DWR
CSB-MP475479DWR
CSB-EP475479DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Ribonuclease P protein component (rnpA) CSB-YP475480DWR
CSB-EP475480DWR
CSB-BP475480DWR
CSB-MP475480DWR
CSB-EP475480DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprothermobacter proteolyticus Alanine racemase (alr) CSB-YP475481DWR
CSB-EP475481DWR
CSB-BP475481DWR
CSB-MP475481DWR
CSB-EP475481DWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Probable DNA ligase (lig), partial CSB-YP475482DKI
CSB-EP475482DKI
CSB-BP475482DKI
CSB-MP475482DKI
CSB-EP475482DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Uracil phosphoribosyltransferase (upp) CSB-YP475483DKI
CSB-EP475483DKI
CSB-BP475483DKI
CSB-MP475483DKI
CSB-EP475483DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Protease HtpX homolog (htpX), partial CSB-YP475484DKI1
CSB-EP475484DKI1
CSB-BP475484DKI1
CSB-MP475484DKI1
CSB-EP475484DKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 60 kDa chaperonin (groL), partial CSB-YP475485DKI
CSB-EP475485DKI
CSB-BP475485DKI
CSB-MP475485DKI
CSB-EP475485DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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