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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 30S ribosomal protein S9 (rpsI) CSB-YP475546EOE
CSB-EP475546EOE
CSB-BP475546EOE
CSB-MP475546EOE
CSB-EP475546EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal protein L11 methyltransferase (prmA) CSB-YP475547EOE
CSB-EP475547EOE
CSB-BP475547EOE
CSB-MP475547EOE
CSB-EP475547EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Outer-membrane lipoprotein carrier protein (lolA) CSB-YP475548EOE
CSB-EP475548EOE
CSB-BP475548EOE
CSB-MP475548EOE
CSB-EP475548EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP475549EOE
CSB-EP475549EOE
CSB-BP475549EOE
CSB-MP475549EOE
CSB-EP475549EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP475550EOE
CSB-EP475550EOE
CSB-BP475550EOE
CSB-MP475550EOE
CSB-EP475550EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Heat shock protein hspQ (hspQ) CSB-YP475551EOE
CSB-EP475551EOE
CSB-BP475551EOE
CSB-MP475551EOE
CSB-EP475551EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S8 (rpsH) CSB-YP475552EOE
CSB-EP475552EOE
CSB-BP475552EOE
CSB-MP475552EOE
CSB-EP475552EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S3 (rpsC) CSB-YP475553EOE
CSB-EP475553EOE
CSB-BP475553EOE
CSB-MP475553EOE
CSB-EP475553EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S7 (rpsG) CSB-YP475554EOE
CSB-EP475554EOE
CSB-BP475554EOE
CSB-MP475554EOE
CSB-EP475554EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutathione-regulated potassium-efflux system ancillary protein KefG (kefG) CSB-YP475555EOE
CSB-EP475555EOE
CSB-BP475555EOE
CSB-MP475555EOE
CSB-EP475555EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Fe/S biogenesis protein NfuA (nfuA) CSB-YP475556EOE
CSB-EP475556EOE
CSB-BP475556EOE
CSB-MP475556EOE
CSB-EP475556EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Curved DNA-binding protein (cbpA) CSB-YP475557EOE
CSB-EP475557EOE
CSB-BP475557EOE
CSB-MP475557EOE
CSB-EP475557EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 ATP phosphoribosyltransferase (hisG) CSB-YP475558EOE
CSB-EP475558EOE
CSB-BP475558EOE
CSB-MP475558EOE
CSB-EP475558EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glycogen synthase (glgA) CSB-YP475559EOE
CSB-EP475559EOE
CSB-BP475559EOE
CSB-MP475559EOE
CSB-EP475559EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Sulfurtransferase TusA (tusA) CSB-YP475560EOE
CSB-EP475560EOE
CSB-BP475560EOE
CSB-MP475560EOE
CSB-EP475560EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Urease accessory protein UreE (ureE) CSB-YP475561EOE
CSB-EP475561EOE
CSB-BP475561EOE
CSB-MP475561EOE
CSB-EP475561EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Hydroxyethylthiazole kinase (thiM) CSB-YP475562EOE
CSB-EP475562EOE
CSB-BP475562EOE
CSB-MP475562EOE
CSB-EP475562EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP475563EOE
CSB-EP475563EOE
CSB-BP475563EOE
CSB-MP475563EOE
CSB-EP475563EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Dihydroorotase (pyrC) CSB-YP475564EOE
CSB-EP475564EOE
CSB-BP475564EOE
CSB-MP475564EOE
CSB-EP475564EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Phosphate acyltransferase (plsX) CSB-YP475565EOE
CSB-EP475565EOE
CSB-BP475565EOE
CSB-MP475565EOE
CSB-EP475565EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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