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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio fischeri Dihydrodipicolinate synthase (dapA) CSB-YP477366VFC
CSB-EP477366VFC
CSB-BP477366VFC
CSB-MP477366VFC
CSB-EP477366VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0246 protein yaaA (yaaA) CSB-YP477367SWN
CSB-EP477367SWN
CSB-BP477367SWN
CSB-MP477367SWN
CSB-EP477367SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein fixA (fixA) CSB-YP477368SWN
CSB-EP477368SWN
CSB-BP477368SWN
CSB-MP477368SWN
CSB-EP477368SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Voltage-gated ClC-type chloride channel ClcB (clcB), partial CSB-YP477369SWN
CSB-EP477369SWN
CSB-BP477369SWN
CSB-MP477369SWN
CSB-EP477369SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Multifunctional CCA protein (cca) CSB-YP477370SWN
CSB-EP477370SWN
CSB-BP477370SWN
CSB-MP477370SWN
CSB-EP477370SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP477371SWN
CSB-EP477371SWN
CSB-BP477371SWN
CSB-MP477371SWN
CSB-EP477371SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosome-binding factor A (rbfA) CSB-YP477372SWN
CSB-EP477372SWN
CSB-BP477372SWN
CSB-MP477372SWN
CSB-EP477372SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP477373SWN
CSB-EP477373SWN
CSB-BP477373SWN
CSB-MP477373SWN
CSB-EP477373SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribulokinase (araB), partial CSB-YP477374SWN
CSB-EP477374SWN
CSB-BP477374SWN
CSB-MP477374SWN
CSB-EP477374SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP477375SWN
CSB-EP477375SWN
CSB-BP477375SWN
CSB-MP477375SWN
CSB-EP477375SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Tyrosine--tRNA ligase (tyrS) CSB-YP477376SWN
CSB-EP477376SWN
CSB-BP477376SWN
CSB-MP477376SWN
CSB-EP477376SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin GTPase obg (obg) CSB-YP477377SWN
CSB-EP477377SWN
CSB-BP477377SWN
CSB-MP477377SWN
CSB-EP477377SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP477378SWN
CSB-EP477378SWN
CSB-BP477378SWN
CSB-MP477378SWN
CSB-EP477378SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Pantothenate synthetase (panC) CSB-YP477379SWN
CSB-EP477379SWN
CSB-BP477379SWN
CSB-MP477379SWN
CSB-EP477379SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Lipid-A-disaccharide synthase (lpxB) CSB-YP477380SWN
CSB-EP477380SWN
CSB-BP477380SWN
CSB-MP477380SWN
CSB-EP477380SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Proline--tRNA ligase (proS), partial CSB-YP477381SWN
CSB-EP477381SWN
CSB-BP477381SWN
CSB-MP477381SWN
CSB-EP477381SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Putative phosphotransferase ydiA (ydiA) CSB-YP477382SWN
CSB-EP477382SWN
CSB-BP477382SWN
CSB-MP477382SWN
CSB-EP477382SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Threonine--tRNA ligase (thrS), partial CSB-YP477383SWN
CSB-EP477383SWN
CSB-BP477383SWN
CSB-MP477383SWN
CSB-EP477383SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Succinylornithine transaminase (astC) CSB-YP477384SWN
CSB-EP477384SWN
CSB-BP477384SWN
CSB-MP477384SWN
CSB-EP477384SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L18 (rplR) CSB-YP477385SWN
CSB-EP477385SWN
CSB-BP477385SWN
CSB-MP477385SWN
CSB-EP477385SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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