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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium leguminosarum bv. trifolii Urease subunit beta (ureB) CSB-YP475666RKQ
CSB-EP475666RKQ
CSB-BP475666RKQ
CSB-MP475666RKQ
CSB-EP475666RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Heat-inducible transcription repressor HrcA (hrcA) CSB-YP475667RKQ
CSB-EP475667RKQ
CSB-BP475667RKQ
CSB-MP475667RKQ
CSB-EP475667RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S2 (rpsB) CSB-YP475668RKQ
CSB-EP475668RKQ
CSB-BP475668RKQ
CSB-MP475668RKQ
CSB-EP475668RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Enolase (eno) CSB-YP475669RKQ
CSB-EP475669RKQ
CSB-BP475669RKQ
CSB-MP475669RKQ
CSB-EP475669RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Phosphoglucosamine mutase (glmM) CSB-YP475670RKQ
CSB-EP475670RKQ
CSB-BP475670RKQ
CSB-MP475670RKQ
CSB-EP475670RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Bifunctional protein GlmU (glmU) CSB-YP475671RKQ
CSB-EP475671RKQ
CSB-BP475671RKQ
CSB-MP475671RKQ
CSB-EP475671RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 50S ribosomal protein L36 (rpmJ) CSB-YP475672RKQ
CSB-EP475672RKQ
CSB-BP475672RKQ
CSB-MP475672RKQ
CSB-EP475672RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0262 protein Rleg2_0240 (Rleg2_0240) CSB-YP475673RKQ
CSB-EP475673RKQ
CSB-BP475673RKQ
CSB-MP475673RKQ
CSB-EP475673RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP475674RKQ
CSB-EP475674RKQ
CSB-BP475674RKQ
CSB-MP475674RKQ
CSB-EP475674RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Probable RNA 2'-phosphotransferase (kptA) CSB-YP475675RKQ
CSB-EP475675RKQ
CSB-BP475675RKQ
CSB-MP475675RKQ
CSB-EP475675RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S4 (rpsD) CSB-YP475676RKQ
CSB-EP475676RKQ
CSB-BP475676RKQ
CSB-MP475676RKQ
CSB-EP475676RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP475677RKQ
CSB-EP475677RKQ
CSB-BP475677RKQ
CSB-MP475677RKQ
CSB-EP475677RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP475678RKQ
CSB-EP475678RKQ
CSB-BP475678RKQ
CSB-MP475678RKQ
CSB-EP475678RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP475679RKQ
CSB-EP475679RKQ
CSB-BP475679RKQ
CSB-MP475679RKQ
CSB-EP475679RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Cobyric acid synthase (cobQ) CSB-YP475680RKQ
CSB-EP475680RKQ
CSB-BP475680RKQ
CSB-MP475680RKQ
CSB-EP475680RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP475681RKQ
CSB-EP475681RKQ
CSB-BP475681RKQ
CSB-MP475681RKQ
CSB-EP475681RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Elongation factor P (efp) CSB-YP475682RKQ
CSB-EP475682RKQ
CSB-BP475682RKQ
CSB-MP475682RKQ
CSB-EP475682RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 50S ribosomal protein L21 (rplU) CSB-YP475683RKQ
CSB-EP475683RKQ
CSB-BP475683RKQ
CSB-MP475683RKQ
CSB-EP475683RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP475684RKQ
CSB-EP475684RKQ
CSB-BP475684RKQ
CSB-MP475684RKQ
CSB-EP475684RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0061 protein Rleg2_0857 (Rleg2_0857) CSB-YP475685RKQ
CSB-EP475685RKQ
CSB-BP475685RKQ
CSB-MP475685RKQ
CSB-EP475685RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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