Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP476466BQP
CSB-EP476466BQP
CSB-BP476466BQP
CSB-MP476466BQP
CSB-EP476466BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphopantetheine adenylyltransferase (coaD) CSB-YP476467BQP
CSB-EP476467BQP
CSB-BP476467BQP
CSB-MP476467BQP
CSB-EP476467BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0435 protein BCG9842_B4853 (BCG9842_B4853) CSB-YP476468BQP
CSB-EP476468BQP
CSB-BP476468BQP
CSB-MP476468BQP
CSB-EP476468BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylmalonate semialdehyde dehydrogenase [acylating] (iolA) CSB-YP476469BQP
CSB-EP476469BQP
CSB-BP476469BQP
CSB-MP476469BQP
CSB-EP476469BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP476470BQP
CSB-EP476470BQP
CSB-BP476470BQP
CSB-MP476470BQP
CSB-EP476470BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease Z (rnz) CSB-YP476471BQP
CSB-EP476471BQP
CSB-BP476471BQP
CSB-MP476471BQP
CSB-EP476471BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP476472BQP
CSB-EP476472BQP
CSB-BP476472BQP
CSB-MP476472BQP
CSB-EP476472BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor P (efp) CSB-YP476473BQP
CSB-EP476473BQP
CSB-BP476473BQP
CSB-MP476473BQP
CSB-EP476473BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP476474BQP
CSB-EP476474BQP
CSB-BP476474BQP
CSB-MP476474BQP
CSB-EP476474BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP476475BQP
CSB-EP476475BQP
CSB-BP476475BQP
CSB-MP476475BQP
CSB-EP476475BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable rRNA maturation factor (BCG9842_B0816) CSB-YP476476BQP
CSB-EP476476BQP
CSB-BP476476BQP
CSB-MP476476BQP
CSB-EP476476BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein grpE (grpE) CSB-YP476477BQP
CSB-EP476477BQP
CSB-BP476477BQP
CSB-MP476477BQP
CSB-EP476477BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Shikimate dehydrogenase (aroE) CSB-YP476478BQP
CSB-EP476478BQP
CSB-BP476478BQP
CSB-MP476478BQP
CSB-EP476478BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0296 protein BCG9842_A0044 (BCG9842_A0044) CSB-YP476479BQP
CSB-EP476479BQP
CSB-BP476479BQP
CSB-MP476479BQP
CSB-EP476479BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Threonine--tRNA ligase (thrS), partial CSB-YP476480BUG
CSB-EP476480BUG
CSB-BP476480BUG
CSB-MP476480BUG
CSB-EP476480BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L21 (rplU) CSB-YP476481BUG
CSB-EP476481BUG
CSB-BP476481BUG
CSB-MP476481BUG
CSB-EP476481BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Peptidyl-tRNA hydrolase (pth) CSB-YP476482BUG
CSB-EP476482BUG
CSB-BP476482BUG
CSB-MP476482BUG
CSB-EP476482BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi tRNA pseudouridine synthase B (truB) CSB-YP476483BUG
CSB-EP476483BUG
CSB-BP476483BUG
CSB-MP476483BUG
CSB-EP476483BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi UvrABC system protein B (uvrB), partial CSB-YP476484BUG
CSB-EP476484BUG
CSB-BP476484BUG
CSB-MP476484BUG
CSB-EP476484BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi UPF0178 protein BbuZS7_0029 (BbuZS7_0029) CSB-YP476485BUG
CSB-EP476485BUG
CSB-BP476485BUG
CSB-MP476485BUG
CSB-EP476485BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>