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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella heidelberg Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP476866SWQ
CSB-EP476866SWQ
CSB-BP476866SWQ
CSB-MP476866SWQ
CSB-EP476866SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Cytochrome c-552 (nrfA) CSB-YP476867SWQ
CSB-EP476867SWQ
CSB-BP476867SWQ
CSB-MP476867SWQ
CSB-EP476867SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable phosphatase YcdX (ycdX) CSB-YP476868SWQ
CSB-EP476868SWQ
CSB-BP476868SWQ
CSB-MP476868SWQ
CSB-EP476868SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Multidrug resistance protein MdtH (mdtH), partial CSB-YP476869SWQ
CSB-EP476869SWQ
CSB-BP476869SWQ
CSB-MP476869SWQ
CSB-EP476869SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Protein RecA (recA) CSB-YP476870SWQ
CSB-EP476870SWQ
CSB-BP476870SWQ
CSB-MP476870SWQ
CSB-EP476870SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 60 kDa chaperonin (groL), partial CSB-YP476871SWQ
CSB-EP476871SWQ
CSB-BP476871SWQ
CSB-MP476871SWQ
CSB-EP476871SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Primosomal replication protein n (priB) CSB-YP476872SWQ
CSB-EP476872SWQ
CSB-BP476872SWQ
CSB-MP476872SWQ
CSB-EP476872SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Peptidase T (pepT) CSB-YP476873SWQ
CSB-EP476873SWQ
CSB-BP476873SWQ
CSB-MP476873SWQ
CSB-EP476873SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP476874SWQ
CSB-EP476874SWQ
CSB-BP476874SWQ
CSB-MP476874SWQ
CSB-EP476874SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP476875SWQ
CSB-EP476875SWQ
CSB-BP476875SWQ
CSB-MP476875SWQ
CSB-EP476875SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0386 protein yjhX (yjhX) CSB-YP476876SWQ
CSB-EP476876SWQ
CSB-BP476876SWQ
CSB-MP476876SWQ
CSB-EP476876SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg N-succinylarginine dihydrolase (astB) CSB-YP476877SWQ
CSB-EP476877SWQ
CSB-BP476877SWQ
CSB-MP476877SWQ
CSB-EP476877SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Threonine--tRNA ligase (thrS), partial CSB-YP476878SWQ
CSB-EP476878SWQ
CSB-BP476878SWQ
CSB-MP476878SWQ
CSB-EP476878SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Integration host factor subunit alpha (ihfA) CSB-YP476879SWQ
CSB-EP476879SWQ
CSB-BP476879SWQ
CSB-MP476879SWQ
CSB-EP476879SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg L-fucose isomerase (fucI), partial CSB-YP476880SWQ
CSB-EP476880SWQ
CSB-BP476880SWQ
CSB-MP476880SWQ
CSB-EP476880SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Lysophospholipid transporter lplT (lplT), partial CSB-YP476881SWQ
CSB-EP476881SWQ
CSB-BP476881SWQ
CSB-MP476881SWQ
CSB-EP476881SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg tRNA-modifying protein ygfZ (ygfZ) CSB-YP476882SWQ
CSB-EP476882SWQ
CSB-BP476882SWQ
CSB-MP476882SWQ
CSB-EP476882SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Deoxyribose-phosphate aldolase (deoC) CSB-YP476883SWQ
CSB-EP476883SWQ
CSB-BP476883SWQ
CSB-MP476883SWQ
CSB-EP476883SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Non-canonical purine NTP phosphatase (yjjX) CSB-YP476884SWQ
CSB-EP476884SWQ
CSB-BP476884SWQ
CSB-MP476884SWQ
CSB-EP476884SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Tyrosine--tRNA ligase (tyrS) CSB-YP476885SWQ
CSB-EP476885SWQ
CSB-BP476885SWQ
CSB-MP476885SWQ
CSB-EP476885SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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