Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Membrane protein insertase YidC (yidC), partial CSB-YP476066DPP1
CSB-EP476066DPP1
CSB-BP476066DPP1
CSB-MP476066DPP1
CSB-EP476066DPP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (CFPG_267) CSB-YP476067DPP
CSB-EP476067DPP
CSB-BP476067DPP
CSB-MP476067DPP
CSB-EP476067DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase 1 (miaA1) CSB-YP476068DPP
CSB-EP476068DPP
CSB-BP476068DPP
CSB-MP476068DPP
CSB-EP476068DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S20 (rpsT) CSB-YP476069DPP
CSB-EP476069DPP
CSB-BP476069DPP
CSB-MP476069DPP
CSB-EP476069DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP476070DPP
CSB-EP476070DPP
CSB-BP476070DPP
CSB-MP476070DPP
CSB-EP476070DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome maturation factor RimM (rimM) CSB-YP476071DPP
CSB-EP476071DPP
CSB-BP476071DPP
CSB-MP476071DPP
CSB-EP476071DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP476072DPP
CSB-EP476072DPP
CSB-BP476072DPP
CSB-MP476072DPP
CSB-EP476072DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP476073DPP
CSB-EP476073DPP
CSB-BP476073DPP
CSB-MP476073DPP
CSB-EP476073DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP476074DPP
CSB-EP476074DPP
CSB-BP476074DPP
CSB-MP476074DPP
CSB-EP476074DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP476075DPP
CSB-EP476075DPP
CSB-BP476075DPP
CSB-MP476075DPP
CSB-EP476075DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S16 (rpsP) CSB-YP476076DPP
CSB-EP476076DPP
CSB-BP476076DPP
CSB-MP476076DPP
CSB-EP476076DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP476077DPP
CSB-EP476077DPP
CSB-BP476077DPP
CSB-MP476077DPP
CSB-EP476077DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP476078DPP
CSB-EP476078DPP
CSB-BP476078DPP
CSB-MP476078DPP
CSB-EP476078DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP476079TMR
CSB-EP476079TMR
CSB-BP476079TMR
CSB-MP476079TMR
CSB-EP476079TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S19P (rps19p) CSB-YP476080TMR
CSB-EP476080TMR
CSB-BP476080TMR
CSB-MP476080TMR
CSB-EP476080TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S8P (rps8p) CSB-YP476081TMR
CSB-EP476081TMR
CSB-BP476081TMR
CSB-MP476081TMR
CSB-EP476081TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable tRNA pseudouridine synthase B (truB) CSB-YP476082TMR
CSB-EP476082TMR
CSB-BP476082TMR
CSB-MP476082TMR
CSB-EP476082TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-directed RNA polymerase subunit K (rpoK) CSB-YP476083TMR
CSB-EP476083TMR
CSB-BP476083TMR
CSB-MP476083TMR
CSB-EP476083TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Tryptophan synthase beta chain (trpB) CSB-YP476084TMR
CSB-EP476084TMR
CSB-BP476084TMR
CSB-MP476084TMR
CSB-EP476084TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Preprotein translocase subunit secE (secE), partial CSB-YP476085TMR
CSB-EP476085TMR
CSB-BP476085TMR
CSB-MP476085TMR
CSB-EP476085TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>