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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida Peptidyl-tRNA hydrolase (pth) CSB-YP475746AZM
CSB-EP475746AZM
CSB-BP475746AZM
CSB-MP475746AZM
CSB-EP475746AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Chaperone protein htpG (htpG), partial CSB-YP475747AZM
CSB-EP475747AZM
CSB-BP475747AZM
CSB-MP475747AZM
CSB-EP475747AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Putative transport protein VSAL_I2029 (VSAL_I2029), partial CSB-YP475748AZM
CSB-EP475748AZM
CSB-BP475748AZM
CSB-MP475748AZM
CSB-EP475748AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UvrABC system protein C (uvrC), partial CSB-YP475749AZM
CSB-EP475749AZM
CSB-BP475749AZM
CSB-MP475749AZM
CSB-EP475749AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Na (+)/H (+) antiporter nhaB, partial CSB-YP475750AZM
CSB-EP475750AZM
CSB-BP475750AZM
CSB-MP475750AZM
CSB-EP475750AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Leucine--tRNA ligase (leuS), partial CSB-YP475751AZM
CSB-EP475751AZM
CSB-BP475751AZM
CSB-MP475751AZM
CSB-EP475751AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0745 protein VSAL_I1068 (VSAL_I1068) CSB-YP475752AZM
CSB-EP475752AZM
CSB-BP475752AZM
CSB-MP475752AZM
CSB-EP475752AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP475753AZM
CSB-EP475753AZM
CSB-BP475753AZM
CSB-MP475753AZM
CSB-EP475753AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L32 (rpmF) CSB-YP475754AZM
CSB-EP475754AZM
CSB-BP475754AZM
CSB-MP475754AZM
CSB-EP475754AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Deoxyguanosinetriphosphate triphosphohydrolase-like protein (VSAL_I1091) CSB-YP475755AZM
CSB-EP475755AZM
CSB-BP475755AZM
CSB-MP475755AZM
CSB-EP475755AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP475756AZM
CSB-EP475756AZM
CSB-BP475756AZM
CSB-MP475756AZM
CSB-EP475756AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida DNA ligase (ligA), partial CSB-YP475757AZM
CSB-EP475757AZM
CSB-BP475757AZM
CSB-MP475757AZM
CSB-EP475757AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribonuclease HII (rnhB) CSB-YP475758AZM
CSB-EP475758AZM
CSB-BP475758AZM
CSB-MP475758AZM
CSB-EP475758AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Protease HtpX (htpX), partial CSB-YP475759AZM1
CSB-EP475759AZM1
CSB-BP475759AZM1
CSB-MP475759AZM1
CSB-EP475759AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP475760AZM
CSB-EP475760AZM
CSB-BP475760AZM
CSB-MP475760AZM
CSB-EP475760AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0317 protein VSAL_I1435 (VSAL_I1435) CSB-YP475761AZM
CSB-EP475761AZM
CSB-BP475761AZM
CSB-MP475761AZM
CSB-EP475761AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0231 protein VSAL_I2591 (VSAL_I2591) CSB-YP475762AZM
CSB-EP475762AZM
CSB-BP475762AZM
CSB-MP475762AZM
CSB-EP475762AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Elongation factor P-like protein (VSAL_I1536) CSB-YP475763AZM
CSB-EP475763AZM
CSB-BP475763AZM
CSB-MP475763AZM
CSB-EP475763AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP475764AZM
CSB-EP475764AZM
CSB-BP475764AZM
CSB-MP475764AZM
CSB-EP475764AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0289 protein VSAL_I2634 (VSAL_I2634) CSB-YP475765AZM
CSB-EP475765AZM
CSB-BP475765AZM
CSB-MP475765AZM
CSB-EP475765AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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