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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP477466SWO
CSB-EP477466SWO
CSB-BP477466SWO
CSB-MP477466SWO
CSB-EP477466SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Autonomous glycyl radical cofactor (grcA) CSB-YP477467SWO
CSB-EP477467SWO
CSB-BP477467SWO
CSB-MP477467SWO
CSB-EP477467SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 7-cyano-7-deazaguanine synthase (queC) CSB-YP477468SWO
CSB-EP477468SWO
CSB-BP477468SWO
CSB-MP477468SWO
CSB-EP477468SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Recombination protein RecR (recR) CSB-YP477469SWO
CSB-EP477469SWO
CSB-BP477469SWO
CSB-MP477469SWO
CSB-EP477469SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Spermidine export protein MdtI (mdtI), partial CSB-YP477470SWO1
CSB-EP477470SWO1
CSB-BP477470SWO1
CSB-MP477470SWO1
CSB-EP477470SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 SsrA-binding protein (smpB) CSB-YP477471SWO
CSB-EP477471SWO
CSB-BP477471SWO
CSB-MP477471SWO
CSB-EP477471SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional regulator yidZ (yidZ) CSB-YP477472SWO
CSB-EP477472SWO
CSB-BP477472SWO
CSB-MP477472SWO
CSB-EP477472SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Electron transport complex protein RnfA (rnfA), partial CSB-YP477473SWO1
CSB-EP477473SWO1
CSB-BP477473SWO1
CSB-MP477473SWO1
CSB-EP477473SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional repressor PurR (purR) CSB-YP477474SWO
CSB-EP477474SWO
CSB-BP477474SWO
CSB-MP477474SWO
CSB-EP477474SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Regulatory protein recX (recX) CSB-YP477475SWO
CSB-EP477475SWO
CSB-BP477475SWO
CSB-MP477475SWO
CSB-EP477475SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 4-alpha-L-fucosyltransferase (wecF) CSB-YP477476SWO
CSB-EP477476SWO
CSB-BP477476SWO
CSB-MP477476SWO
CSB-EP477476SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP477477SWO
CSB-EP477477SWO
CSB-BP477477SWO
CSB-MP477477SWO
CSB-EP477477SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP477478SWO
CSB-EP477478SWO
CSB-BP477478SWO
CSB-MP477478SWO
CSB-EP477478SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP477479SWO
CSB-EP477479SWO
CSB-BP477479SWO
CSB-MP477479SWO
CSB-EP477479SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP477480SWO
CSB-EP477480SWO
CSB-BP477480SWO
CSB-MP477480SWO
CSB-EP477480SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP477481SWO
CSB-EP477481SWO
CSB-BP477481SWO
CSB-MP477481SWO
CSB-EP477481SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Potassium-transporting ATPase A chain (kdpA), partial CSB-YP477482SWO
CSB-EP477482SWO
CSB-BP477482SWO
CSB-MP477482SWO
CSB-EP477482SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Cell division activator CedA (cedA) CSB-YP477483SWO
CSB-EP477483SWO
CSB-BP477483SWO
CSB-MP477483SWO
CSB-EP477483SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Succinylornithine transaminase (astC) CSB-YP477484SWO
CSB-EP477484SWO
CSB-BP477484SWO
CSB-MP477484SWO
CSB-EP477484SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 DNA mismatch repair protein mutH (mutH) CSB-YP477485SWO
CSB-EP477485SWO
CSB-BP477485SWO
CSB-MP477485SWO
CSB-EP477485SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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