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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli UPF0235 protein yggU (yggU) CSB-YP475866ENW
CSB-EP475866ENW
CSB-BP475866ENW
CSB-MP475866ENW
CSB-EP475866ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP475867ENW
CSB-EP475867ENW
CSB-BP475867ENW
CSB-MP475867ENW
CSB-EP475867ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP475868ENW1
CSB-EP475868ENW1
CSB-BP475868ENW1
CSB-MP475868ENW1
CSB-EP475868ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0194 membrane protein ybhG (ybhG) CSB-YP475869ENW
CSB-EP475869ENW
CSB-BP475869ENW
CSB-MP475869ENW
CSB-EP475869ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cobalamin synthase (cobS), partial CSB-YP475870ENW1
CSB-EP475870ENW1
CSB-BP475870ENW1
CSB-MP475870ENW1
CSB-EP475870ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP475871ENW
CSB-EP475871ENW
CSB-BP475871ENW
CSB-MP475871ENW
CSB-EP475871ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP475872ENW
CSB-EP475872ENW
CSB-BP475872ENW
CSB-MP475872ENW
CSB-EP475872ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP475873ENW
CSB-EP475873ENW
CSB-BP475873ENW
CSB-MP475873ENW
CSB-EP475873ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP475874ENW
CSB-EP475874ENW
CSB-BP475874ENW
CSB-MP475874ENW
CSB-EP475874ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) CSB-YP475875ENW
CSB-EP475875ENW
CSB-BP475875ENW
CSB-MP475875ENW
CSB-EP475875ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Alkanesulfonate monooxygenase (ssuD) CSB-YP475876ENW
CSB-EP475876ENW
CSB-BP475876ENW
CSB-MP475876ENW
CSB-EP475876ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0319 protein YccT (yccT) CSB-YP475877ENW
CSB-EP475877ENW
CSB-BP475877ENW
CSB-MP475877ENW
CSB-EP475877ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable phosphatase YcdX (ycdX) CSB-YP475878ENW
CSB-EP475878ENW
CSB-BP475878ENW
CSB-MP475878ENW
CSB-EP475878ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug resistance protein MdtG (mdtG), partial CSB-YP475879ENW
CSB-EP475879ENW
CSB-BP475879ENW
CSB-MP475879ENW
CSB-EP475879ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar L-ring protein (flgH) CSB-YP475880ENW
CSB-EP475880ENW
CSB-BP475880ENW
CSB-MP475880ENW
CSB-EP475880ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0227 protein ycfP (ycfP) CSB-YP475881ENW
CSB-EP475881ENW
CSB-BP475881ENW
CSB-MP475881ENW
CSB-EP475881ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0745 protein YcgL (ycgL) CSB-YP475882ENW
CSB-EP475882ENW
CSB-BP475882ENW
CSB-MP475882ENW
CSB-EP475882ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP475883ENW
CSB-EP475883ENW
CSB-BP475883ENW
CSB-MP475883ENW
CSB-EP475883ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable intracellular septation protein A (yciB), partial CSB-YP475884ENW1
CSB-EP475884ENW1
CSB-BP475884ENW1
CSB-MP475884ENW1
CSB-EP475884ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Altronate oxidoreductase (uxaB) CSB-YP475885ENW
CSB-EP475885ENW
CSB-BP475885ENW
CSB-MP475885ENW
CSB-EP475885ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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