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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum Electron transport complex protein RnfE (rnfE), partial CSB-YP477566SWP1
CSB-EP477566SWP1
CSB-BP477566SWP1
CSB-MP477566SWP1
CSB-EP477566SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Quinate/shikimate dehydrogenase (ydiB) CSB-YP477567SWP
CSB-EP477567SWP
CSB-BP477567SWP
CSB-MP477567SWP
CSB-EP477567SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Succinylornithine transaminase (astC) CSB-YP477568SWP
CSB-EP477568SWP
CSB-BP477568SWP
CSB-MP477568SWP
CSB-EP477568SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0227 protein ycfP (ycfP) CSB-YP477569SWP
CSB-EP477569SWP
CSB-BP477569SWP
CSB-MP477569SWP
CSB-EP477569SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum O-acetyl-ADP-ribose deacetylase (ymdB) CSB-YP477570SWP
CSB-EP477570SWP
CSB-BP477570SWP
CSB-MP477570SWP
CSB-EP477570SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP477571SWP
CSB-EP477571SWP
CSB-BP477571SWP
CSB-MP477571SWP
CSB-EP477571SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Histidinol-phosphate aminotransferase (hisC) CSB-YP477572SWP
CSB-EP477572SWP
CSB-BP477572SWP
CSB-MP477572SWP
CSB-EP477572SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Probable lipid kinase YegS (yegS) CSB-YP477573SWP
CSB-EP477573SWP
CSB-BP477573SWP
CSB-MP477573SWP
CSB-EP477573SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP477574SWP
CSB-EP477574SWP
CSB-BP477574SWP
CSB-MP477574SWP
CSB-EP477574SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP477575SWP
CSB-EP477575SWP
CSB-BP477575SWP
CSB-MP477575SWP
CSB-EP477575SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP477576SWP
CSB-EP477576SWP
CSB-BP477576SWP
CSB-MP477576SWP
CSB-EP477576SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Manganese transport protein MntH (mntH), partial CSB-YP477577SWP
CSB-EP477577SWP
CSB-BP477577SWP
CSB-MP477577SWP
CSB-EP477577SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP477578SWP
CSB-EP477578SWP
CSB-BP477578SWP
CSB-MP477578SWP
CSB-EP477578SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum GTPase Der (der) CSB-YP477579SWP
CSB-EP477579SWP
CSB-BP477579SWP
CSB-MP477579SWP
CSB-EP477579SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum tRNA (guanine-N (1)-)-methyltransferase CSB-YP477580SWP
CSB-EP477580SWP
CSB-BP477580SWP
CSB-MP477580SWP
CSB-EP477580SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein RecA (recA) CSB-YP477581SWP
CSB-EP477581SWP
CSB-BP477581SWP
CSB-MP477581SWP
CSB-EP477581SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 5'/3'-nucleotidase SurE (surE) CSB-YP477582SWP
CSB-EP477582SWP
CSB-BP477582SWP
CSB-MP477582SWP
CSB-EP477582SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP477583SWP
CSB-EP477583SWP
CSB-BP477583SWP
CSB-MP477583SWP
CSB-EP477583SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP477584SWP
CSB-EP477584SWP
CSB-BP477584SWP
CSB-MP477584SWP
CSB-EP477584SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Arginine exporter protein ArgO (argO), partial CSB-YP477585SWP1
CSB-EP477585SWP1
CSB-BP477585SWP1
CSB-MP477585SWP1
CSB-EP477585SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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