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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oligotropha carboxidovorans Bifunctional protein GlmU (glmU) CSB-YP475986OED
CSB-EP475986OED
CSB-BP475986OED
CSB-MP475986OED
CSB-EP475986OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP475987OED
CSB-EP475987OED
CSB-BP475987OED
CSB-MP475987OED
CSB-EP475987OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Enolase (eno) CSB-YP475988OED
CSB-EP475988OED
CSB-BP475988OED
CSB-MP475988OED
CSB-EP475988OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Large-conductance mechanosensitive channel (mscL), partial CSB-YP475989OED1
CSB-EP475989OED1
CSB-BP475989OED1
CSB-MP475989OED1
CSB-EP475989OED1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans DNA repair protein recO (recO) CSB-YP475990OED
CSB-EP475990OED
CSB-BP475990OED
CSB-MP475990OED
CSB-EP475990OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Integration host factor subunit alpha (ihfA) CSB-YP475991OED
CSB-EP475991OED
CSB-BP475991OED
CSB-MP475991OED
CSB-EP475991OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP475992OED
CSB-EP475992OED
CSB-BP475992OED
CSB-MP475992OED
CSB-EP475992OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Shikimate dehydrogenase (aroE) CSB-YP475993OED
CSB-EP475993OED
CSB-BP475993OED
CSB-MP475993OED
CSB-EP475993OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP475994OED
CSB-EP475994OED
CSB-BP475994OED
CSB-MP475994OED
CSB-EP475994OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans UPF0317 protein OCAR_7359/OCA5_c07590 (OCAR_7359, OCA5_c07590) CSB-YP475995OED
CSB-EP475995OED
CSB-BP475995OED
CSB-MP475995OED
CSB-EP475995OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP475996OED
CSB-EP475996OED
CSB-BP475996OED
CSB-MP475996OED
CSB-EP475996OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Elongation factor 4 (lepA), partial CSB-YP475997OED
CSB-EP475997OED
CSB-BP475997OED
CSB-MP475997OED
CSB-EP475997OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP475998HUY
CSB-EP475998HUY
CSB-BP475998HUY
CSB-MP475998HUY
CSB-EP475998HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Flagellar P-ring protein (flgI) CSB-YP475999HUY
CSB-EP475999HUY
CSB-BP475999HUY
CSB-MP475999HUY
CSB-EP475999HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L21 (rplU) CSB-YP476000HUY
CSB-EP476000HUY
CSB-BP476000HUY
CSB-MP476000HUY
CSB-EP476000HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Arginine--tRNA ligase (argS) CSB-YP476001HUY
CSB-EP476001HUY
CSB-BP476001HUY
CSB-MP476001HUY
CSB-EP476001HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP476002HUY
CSB-EP476002HUY
CSB-BP476002HUY
CSB-MP476002HUY
CSB-EP476002HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S15 (rpsO) CSB-YP476003HUY
CSB-EP476003HUY
CSB-BP476003HUY
CSB-MP476003HUY
CSB-EP476003HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP476004HUY
CSB-EP476004HUY
CSB-BP476004HUY
CSB-MP476004HUY
CSB-EP476004HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP476005HUY
CSB-EP476005HUY
CSB-BP476005HUY
CSB-MP476005HUY
CSB-EP476005HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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