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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori Urease accessory protein UreH (ureH) CSB-YP476026HUY
CSB-EP476026HUY
CSB-BP476026HUY
CSB-MP476026HUY
CSB-EP476026HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phosphoglucosamine mutase (glmM) CSB-YP476027HUY
CSB-EP476027HUY
CSB-BP476027HUY
CSB-MP476027HUY
CSB-EP476027HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Chaperone protein DnaK (dnaK), partial CSB-YP476028HUY
CSB-EP476028HUY
CSB-BP476028HUY
CSB-MP476028HUY
CSB-EP476028HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Shikimate kinase (aroK) CSB-YP476029HUY
CSB-EP476029HUY
CSB-BP476029HUY
CSB-MP476029HUY
CSB-EP476029HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Amino-acid acetyltransferase, mitochondrial (arg2), partial CSB-YP476030SXT
CSB-EP476030SXT
CSB-BP476030SXT
CSB-MP476030SXT
CSB-EP476030SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Protein get1 (get1), partial CSB-YP476031SXT1
CSB-EP476031SXT1
CSB-BP476031SXT1
CSB-MP476031SXT1
CSB-EP476031SXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus 40S ribosomal protein S0-B (rps0b) CSB-YP476032SXT
CSB-EP476032SXT
CSB-BP476032SXT
CSB-MP476032SXT
CSB-EP476032SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Elongation factor Ts, mitochondrial (tsf1) CSB-YP476033SXT
CSB-EP476033SXT
CSB-BP476033SXT
CSB-MP476033SXT
CSB-EP476033SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Mitochondrial distribution and morphology protein 12 (mdm12) CSB-YP476034SXT
CSB-EP476034SXT
CSB-BP476034SXT
CSB-MP476034SXT
CSB-EP476034SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhoptry neck protein 4 (RON4), partial CSB-YP476035TOV
CSB-EP476035TOV
CSB-BP476035TOV
CSB-MP476035TOV
CSB-EP476035TOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae Probable RISC-loading complex subunit BRAFLDRAFT_242885 (BRAFLDRAFT_242885) CSB-YP476036BVY
CSB-EP476036BVY
CSB-BP476036BVY
CSB-MP476036BVY
CSB-EP476036BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Cyanate hydratase (cyn1) CSB-YP476037ETM
CSB-EP476037ETM
CSB-BP476037ETM
CSB-MP476037ETM
CSB-EP476037ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Nucleolar protein 9 (nop9), partial CSB-YP476038ETM
CSB-EP476038ETM
CSB-BP476038ETM
CSB-MP476038ETM
CSB-EP476038ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Fe-S cluster assembly protein dre2 (dre2) CSB-YP476039ETM
CSB-EP476039ETM
CSB-BP476039ETM
CSB-MP476039ETM
CSB-EP476039ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Pentafunctional AROM polypeptide 2 (aroM-2), partial CSB-YP476040ETM
CSB-EP476040ETM
CSB-BP476040ETM
CSB-MP476040ETM
CSB-EP476040ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable Xaa-Pro aminopeptidase P (ampp), partial CSB-YP476041ETM
CSB-EP476041ETM
CSB-BP476041ETM
CSB-MP476041ETM
CSB-EP476041ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Formation of crista junctions protein 1 (fcj1), partial CSB-YP476042ETM1
CSB-EP476042ETM1
CSB-BP476042ETM1
CSB-MP476042ETM1
CSB-EP476042ETM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable beta-galactosidase B (lacB), partial CSB-YP476043ETM
CSB-EP476043ETM
CSB-BP476043ETM
CSB-MP476043ETM
CSB-EP476043ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Maintenance of mitochondrial morphology protein 1 (mmm1), partial CSB-YP476044ETM
CSB-EP476044ETM
CSB-BP476044ETM
CSB-MP476044ETM
CSB-EP476044ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peptide deformylase 1A, chloroplastic (PDF1A) CSB-YP476045OFG
CSB-EP476045OFG
CSB-BP476045OFG
CSB-MP476045OFG
CSB-EP476045OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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