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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelodictyon phaeoclathratiforme Phosphoheptose isomerase (gmhA) CSB-YP476706ETB
CSB-EP476706ETB
CSB-BP476706ETB
CSB-MP476706ETB
CSB-EP476706ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Peptidyl-tRNA hydrolase (pth) CSB-YP476707ETB
CSB-EP476707ETB
CSB-BP476707ETB
CSB-MP476707ETB
CSB-EP476707ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP476708ETB
CSB-EP476708ETB
CSB-BP476708ETB
CSB-MP476708ETB
CSB-EP476708ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Arginine--tRNA ligase (argS), partial CSB-YP476709ETB
CSB-EP476709ETB
CSB-BP476709ETB
CSB-MP476709ETB
CSB-EP476709ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP476710ETB
CSB-EP476710ETB
CSB-BP476710ETB
CSB-MP476710ETB
CSB-EP476710ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Protein translocase subunit SecA (secA), partial CSB-YP476711ETB
CSB-EP476711ETB
CSB-BP476711ETB
CSB-MP476711ETB
CSB-EP476711ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S21 (rpsU) CSB-YP476712FLY
CSB-EP476712FLY
CSB-BP476712FLY
CSB-MP476712FLY
CSB-EP476712FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP476713FLY
CSB-EP476713FLY
CSB-BP476713FLY
CSB-MP476713FLY
CSB-EP476713FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Flagellar brake protein YcgR (ycgR) CSB-YP476714FLY
CSB-EP476714FLY
CSB-BP476714FLY
CSB-MP476714FLY
CSB-EP476714FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia DNA polymerase IV (dinB) CSB-YP476715FLY
CSB-EP476715FLY
CSB-BP476715FLY
CSB-MP476715FLY
CSB-EP476715FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0234 protein Smal_3487 (Smal_3487) CSB-YP476716FLY
CSB-EP476716FLY
CSB-BP476716FLY
CSB-MP476716FLY
CSB-EP476716FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP476717FLY
CSB-EP476717FLY
CSB-BP476717FLY
CSB-MP476717FLY
CSB-EP476717FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 3-dehydroquinate dehydratase (aroQ) CSB-YP476718FLY
CSB-EP476718FLY
CSB-BP476718FLY
CSB-MP476718FLY
CSB-EP476718FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L3 (rplC) CSB-YP476719FLY
CSB-EP476719FLY
CSB-BP476719FLY
CSB-MP476719FLY
CSB-EP476719FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L14 (rplN) CSB-YP476720FLY
CSB-EP476720FLY
CSB-BP476720FLY
CSB-MP476720FLY
CSB-EP476720FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP476721FLY
CSB-EP476721FLY
CSB-BP476721FLY
CSB-MP476721FLY
CSB-EP476721FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S11 (rpsK) CSB-YP476722FLY
CSB-EP476722FLY
CSB-BP476722FLY
CSB-MP476722FLY
CSB-EP476722FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Trigger factor (tig) CSB-YP476723FLY
CSB-EP476723FLY
CSB-BP476723FLY
CSB-MP476723FLY
CSB-EP476723FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0042 nucleotide-binding protein Smal_0950 (Smal_0950) CSB-YP476724FLY
CSB-EP476724FLY
CSB-BP476724FLY
CSB-MP476724FLY
CSB-EP476724FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Putative phosphotransferase Smal_2421 (Smal_2421) CSB-YP476725FLY
CSB-EP476725FLY
CSB-BP476725FLY
CSB-MP476725FLY
CSB-EP476725FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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