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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella agona tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP477246SWK
CSB-EP477246SWK
CSB-BP477246SWK
CSB-MP477246SWK
CSB-EP477246SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP477247SWK
CSB-EP477247SWK
CSB-BP477247SWK
CSB-MP477247SWK
CSB-EP477247SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0271 protein YbgL (ybgL) CSB-YP477248SWK
CSB-EP477248SWK
CSB-BP477248SWK
CSB-MP477248SWK
CSB-EP477248SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Nucleoside triphosphatase nudI (nudI) CSB-YP477249SWK
CSB-EP477249SWK
CSB-BP477249SWK
CSB-MP477249SWK
CSB-EP477249SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP477250SWK
CSB-EP477250SWK
CSB-BP477250SWK
CSB-MP477250SWK
CSB-EP477250SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribonuclease BN (rbn) CSB-YP477251SWK
CSB-EP477251SWK
CSB-BP477251SWK
CSB-MP477251SWK
CSB-EP477251SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP477252SWK
CSB-EP477252SWK
CSB-BP477252SWK
CSB-MP477252SWK
CSB-EP477252SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP477253SWK
CSB-EP477253SWK
CSB-BP477253SWK
CSB-MP477253SWK
CSB-EP477253SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0761 membrane protein yihY (yihY), partial CSB-YP477254SWK1
CSB-EP477254SWK1
CSB-BP477254SWK1
CSB-MP477254SWK1
CSB-EP477254SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP477255SWK
CSB-EP477255SWK
CSB-BP477255SWK
CSB-MP477255SWK
CSB-EP477255SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP477256SWK
CSB-EP477256SWK
CSB-BP477256SWK
CSB-MP477256SWK
CSB-EP477256SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP477257SWK
CSB-EP477257SWK
CSB-BP477257SWK
CSB-MP477257SWK
CSB-EP477257SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cation-efflux pump FieF (fieF), partial CSB-YP477258SWK1
CSB-EP477258SWK1
CSB-BP477258SWK1
CSB-MP477258SWK1
CSB-EP477258SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona ATP-dependent protease subunit HslV (hslV) CSB-YP477259SWK
CSB-EP477259SWK
CSB-BP477259SWK
CSB-MP477259SWK
CSB-EP477259SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L7/L12 (rplL) CSB-YP477260SWK
CSB-EP477260SWK
CSB-BP477260SWK
CSB-MP477260SWK
CSB-EP477260SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP477261SWK
CSB-EP477261SWK
CSB-BP477261SWK
CSB-MP477261SWK
CSB-EP477261SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0434 protein ycaR (ycaR) CSB-YP477262SWK
CSB-EP477262SWK
CSB-BP477262SWK
CSB-MP477262SWK
CSB-EP477262SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP477263SWK
CSB-EP477263SWK
CSB-BP477263SWK
CSB-MP477263SWK
CSB-EP477263SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Iron-binding protein IscA (iscA) CSB-YP477264SWK
CSB-EP477264SWK
CSB-BP477264SWK
CSB-MP477264SWK
CSB-EP477264SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Elongation factor 4 (lepA), partial CSB-YP477265SWK
CSB-EP477265SWK
CSB-BP477265SWK
CSB-MP477265SWK
CSB-EP477265SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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