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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodospirillum centenum Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP478246RLY
CSB-EP478246RLY
CSB-BP478246RLY
CSB-MP478246RLY
CSB-EP478246RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP478247DXM
CSB-EP478247DXM
CSB-BP478247DXM
CSB-MP478247DXM
CSB-EP478247DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP478248DXM
CSB-EP478248DXM
CSB-BP478248DXM
CSB-MP478248DXM
CSB-EP478248DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA repair protein recO (recO) CSB-YP478249DXM
CSB-EP478249DXM
CSB-BP478249DXM
CSB-MP478249DXM
CSB-EP478249DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP478250DXM
CSB-EP478250DXM
CSB-BP478250DXM
CSB-MP478250DXM
CSB-EP478250DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome-binding factor A (rbfA) CSB-YP478251DXM
CSB-EP478251DXM
CSB-BP478251DXM
CSB-MP478251DXM
CSB-EP478251DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphoglucosamine mutase (glmM) CSB-YP478252DXM
CSB-EP478252DXM
CSB-BP478252DXM
CSB-MP478252DXM
CSB-EP478252DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii SsrA-binding protein (smpB) CSB-YP478253DXM
CSB-EP478253DXM
CSB-BP478253DXM
CSB-MP478253DXM
CSB-EP478253DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Histidine--tRNA ligase (hisS) CSB-YP478254DXM
CSB-EP478254DXM
CSB-BP478254DXM
CSB-MP478254DXM
CSB-EP478254DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP478255DXM
CSB-EP478255DXM
CSB-BP478255DXM
CSB-MP478255DXM
CSB-EP478255DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Biotin synthase (bioB) CSB-YP478256DXM
CSB-EP478256DXM
CSB-BP478256DXM
CSB-MP478256DXM
CSB-EP478256DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Dihydroorotate dehydrogenase (quinone) CSB-YP478257DXM
CSB-EP478257DXM
CSB-BP478257DXM
CSB-MP478257DXM
CSB-EP478257DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Peptide chain release factor 3 (prfC), partial CSB-YP478258DXM
CSB-EP478258DXM
CSB-BP478258DXM
CSB-MP478258DXM
CSB-EP478258DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP478259DXM
CSB-EP478259DXM
CSB-BP478259DXM
CSB-MP478259DXM
CSB-EP478259DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP478260DXM
CSB-EP478260DXM
CSB-BP478260DXM
CSB-MP478260DXM
CSB-EP478260DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP478261DXM
CSB-EP478261DXM
CSB-BP478261DXM
CSB-MP478261DXM
CSB-EP478261DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP478262DXM
CSB-EP478262DXM
CSB-BP478262DXM
CSB-MP478262DXM
CSB-EP478262DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Adenylate kinase (adk) CSB-YP478263DXM
CSB-EP478263DXM
CSB-BP478263DXM
CSB-MP478263DXM
CSB-EP478263DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Isoleucine--tRNA ligase (ileS), partial CSB-YP478264DXM
CSB-EP478264DXM
CSB-BP478264DXM
CSB-MP478264DXM
CSB-EP478264DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP478265DXM
CSB-EP478265DXM
CSB-BP478265DXM
CSB-MP478265DXM
CSB-EP478265DXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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