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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus UPF0374 protein BCAH820_0510 (BCAH820_0510) CSB-YP476586BQM
CSB-EP476586BQM
CSB-BP476586BQM
CSB-MP476586BQM
CSB-EP476586BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S20 (rpsT) CSB-YP476587BQM
CSB-EP476587BQM
CSB-BP476587BQM
CSB-MP476587BQM
CSB-EP476587BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable transcriptional regulatory protein BCAH820_0541 (BCAH820_0541) CSB-YP476588BQM
CSB-EP476588BQM
CSB-BP476588BQM
CSB-MP476588BQM
CSB-EP476588BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0297 protein BCAH820_4467 (BCAH820_4467) CSB-YP476589BQM
CSB-EP476589BQM
CSB-BP476589BQM
CSB-MP476589BQM
CSB-EP476589BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Quinolinate synthase A (nadA) CSB-YP476590BQM
CSB-EP476590BQM
CSB-BP476590BQM
CSB-MP476590BQM
CSB-EP476590BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septum formation protein Maf (maf) CSB-YP476591BQM
CSB-EP476591BQM
CSB-BP476591BQM
CSB-MP476591BQM
CSB-EP476591BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease PH (rph) CSB-YP476592BQM
CSB-EP476592BQM
CSB-BP476592BQM
CSB-MP476592BQM
CSB-EP476592BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus MutS2 protein (mutS2), partial CSB-YP476593BQM
CSB-EP476593BQM
CSB-BP476593BQM
CSB-MP476593BQM
CSB-EP476593BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thiazole synthase (thiG) CSB-YP476594BQM
CSB-EP476594BQM
CSB-BP476594BQM
CSB-MP476594BQM
CSB-EP476594BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP476595BQM
CSB-EP476595BQM
CSB-BP476595BQM
CSB-MP476595BQM
CSB-EP476595BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0754 membrane protein BCAH820_0954 (BCAH820_0954), partial CSB-YP476596BQM1
CSB-EP476596BQM1
CSB-BP476596BQM1
CSB-MP476596BQM1
CSB-EP476596BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP476597BQM
CSB-EP476597BQM
CSB-BP476597BQM
CSB-MP476597BQM
CSB-EP476597BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UPF0754 membrane protein PCC8801_0398 (PCC8801_0398), partial CSB-YP476598EPW1
CSB-EP476598EPW1
CSB-BP476598EPW1
CSB-MP476598EPW1
CSB-EP476598EPW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cysteine--tRNA ligase (cysS) CSB-YP476599EPW
CSB-EP476599EPW
CSB-BP476599EPW
CSB-MP476599EPW
CSB-EP476599EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S12 (rpsL) CSB-YP476600EPW
CSB-EP476600EPW
CSB-BP476600EPW
CSB-MP476600EPW
CSB-EP476600EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 33 kDa chaperonin (hslO) CSB-YP476601EPW
CSB-EP476601EPW
CSB-BP476601EPW
CSB-MP476601EPW
CSB-EP476601EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S4 (rpsD) CSB-YP476602EPW
CSB-EP476602EPW
CSB-BP476602EPW
CSB-MP476602EPW
CSB-EP476602EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. GTPase obg (obg) CSB-YP476603EPW
CSB-EP476603EPW
CSB-BP476603EPW
CSB-MP476603EPW
CSB-EP476603EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP476604EPW1
CSB-EP476604EPW1
CSB-BP476604EPW1
CSB-MP476604EPW1
CSB-EP476604EPW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein H (psbH), partial CSB-YP476605EPW1
CSB-EP476605EPW1
CSB-BP476605EPW1
CSB-MP476605EPW1
CSB-EP476605EPW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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