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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stenotrophomonas maltophilia DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP476746FLY
CSB-EP476746FLY
CSB-BP476746FLY
CSB-MP476746FLY
CSB-EP476746FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP476747SWS
CSB-EP476747SWS
CSB-BP476747SWS
CSB-MP476747SWS
CSB-EP476747SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0289 protein yacF (yacF) CSB-YP476748SWS
CSB-EP476748SWS
CSB-BP476748SWS
CSB-MP476748SWS
CSB-EP476748SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP476749SWS
CSB-EP476749SWS
CSB-BP476749SWS
CSB-MP476749SWS
CSB-EP476749SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Peptidyl-tRNA hydrolase (pth) CSB-YP476750SWS
CSB-EP476750SWS
CSB-BP476750SWS
CSB-MP476750SWS
CSB-EP476750SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Probable septum site-determining protein MinC (minC) CSB-YP476751SWS
CSB-EP476751SWS
CSB-BP476751SWS
CSB-MP476751SWS
CSB-EP476751SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP476752SWS1
CSB-EP476752SWS1
CSB-BP476752SWS1
CSB-MP476752SWS1
CSB-EP476752SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 50S ribosomal protein L18 (rplR) CSB-YP476753SWS
CSB-EP476753SWS
CSB-BP476753SWS
CSB-MP476753SWS
CSB-EP476753SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 30S ribosomal protein S17 (rpsQ) CSB-YP476754SWS
CSB-EP476754SWS
CSB-BP476754SWS
CSB-MP476754SWS
CSB-EP476754SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 50S ribosomal protein L3 (rplC) CSB-YP476755SWS
CSB-EP476755SWS
CSB-BP476755SWS
CSB-MP476755SWS
CSB-EP476755SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Sulfurtransferase TusD (tusD) CSB-YP476756SWS
CSB-EP476756SWS
CSB-BP476756SWS
CSB-MP476756SWS
CSB-EP476756SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0325 protein yaeH (yaeH) CSB-YP476757SWS
CSB-EP476757SWS
CSB-BP476757SWS
CSB-MP476757SWS
CSB-EP476757SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP476758SWS
CSB-EP476758SWS
CSB-BP476758SWS
CSB-MP476758SWS
CSB-EP476758SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0294 protein yafD (yafD) CSB-YP476759SWS
CSB-EP476759SWS
CSB-BP476759SWS
CSB-MP476759SWS
CSB-EP476759SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP476760SWS
CSB-EP476760SWS
CSB-BP476760SWS
CSB-MP476760SWS
CSB-EP476760SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Aspartate--tRNA ligase (aspS), partial CSB-YP476761SWS
CSB-EP476761SWS
CSB-BP476761SWS
CSB-MP476761SWS
CSB-EP476761SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP476762SWS
CSB-EP476762SWS
CSB-BP476762SWS
CSB-MP476762SWS
CSB-EP476762SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Esterase FrsA (frsA) CSB-YP476763SWS
CSB-EP476763SWS
CSB-BP476763SWS
CSB-MP476763SWS
CSB-EP476763SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Anti-adapter protein iraP (iraP) CSB-YP476764SWS
CSB-EP476764SWS
CSB-BP476764SWS
CSB-MP476764SWS
CSB-EP476764SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Flagellar hook-basal body complex protein FliE (fliE) CSB-YP476765SWS
CSB-EP476765SWS
CSB-BP476765SWS
CSB-MP476765SWS
CSB-EP476765SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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