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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella newport DNA ligase (ligA), partial CSB-YP476786SWS
CSB-EP476786SWS
CSB-BP476786SWS
CSB-MP476786SWS
CSB-EP476786SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport L-rhamnose mutarotase (rhaM) CSB-YP476787SWS
CSB-EP476787SWS
CSB-BP476787SWS
CSB-MP476787SWS
CSB-EP476787SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport DNA protection during starvation protein (dps) CSB-YP476788SWS
CSB-EP476788SWS
CSB-BP476788SWS
CSB-MP476788SWS
CSB-EP476788SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP476789SWS
CSB-EP476789SWS
CSB-BP476789SWS
CSB-MP476789SWS
CSB-EP476789SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP476790SWS
CSB-EP476790SWS
CSB-BP476790SWS
CSB-MP476790SWS
CSB-EP476790SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Histidine--tRNA ligase (hisS) CSB-YP476791SWS
CSB-EP476791SWS
CSB-BP476791SWS
CSB-MP476791SWS
CSB-EP476791SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Peptidase B (pepB) CSB-YP476792SWS
CSB-EP476792SWS
CSB-BP476792SWS
CSB-MP476792SWS
CSB-EP476792SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Cell division protein ZapB (zapB) CSB-YP476793SWS
CSB-EP476793SWS
CSB-BP476793SWS
CSB-MP476793SWS
CSB-EP476793SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Pantothenate kinase (coaA) CSB-YP476794SWS
CSB-EP476794SWS
CSB-BP476794SWS
CSB-MP476794SWS
CSB-EP476794SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Thiamine-phosphate synthase (thiE) CSB-YP476795SWS
CSB-EP476795SWS
CSB-BP476795SWS
CSB-MP476795SWS
CSB-EP476795SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Serine--tRNA ligase (serS) CSB-YP476796SWS
CSB-EP476796SWS
CSB-BP476796SWS
CSB-MP476796SWS
CSB-EP476796SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP476797SWS
CSB-EP476797SWS
CSB-BP476797SWS
CSB-MP476797SWS
CSB-EP476797SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport HTH-type transcriptional regulator iscR (iscR) CSB-YP476798SWS
CSB-EP476798SWS
CSB-BP476798SWS
CSB-MP476798SWS
CSB-EP476798SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP476799SWS
CSB-EP476799SWS
CSB-BP476799SWS
CSB-MP476799SWS
CSB-EP476799SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Heat shock protein hspQ (hspQ) CSB-YP476800SWS
CSB-EP476800SWS
CSB-BP476800SWS
CSB-MP476800SWS
CSB-EP476800SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Fumarate reductase subunit D (frdD), partial CSB-YP476801SWS1
CSB-EP476801SWS1
CSB-BP476801SWS1
CSB-MP476801SWS1
CSB-EP476801SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Adenylosuccinate synthetase (purA) CSB-YP476802SWS
CSB-EP476802SWS
CSB-BP476802SWS
CSB-MP476802SWS
CSB-EP476802SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Probable phosphatase YcdX (ycdX) CSB-YP476803SWS
CSB-EP476803SWS
CSB-BP476803SWS
CSB-MP476803SWS
CSB-EP476803SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport N-methyl-L-tryptophan oxidase (solA) CSB-YP476804SWS
CSB-EP476804SWS
CSB-BP476804SWS
CSB-MP476804SWS
CSB-EP476804SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Phosphate acyltransferase (plsX) CSB-YP476805SWS
CSB-EP476805SWS
CSB-BP476805SWS
CSB-MP476805SWS
CSB-EP476805SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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