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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin Probable rRNA maturation factor YbeY (ybeY) CSB-YP477406SWN
CSB-EP477406SWN
CSB-BP477406SWN
CSB-MP477406SWN
CSB-EP477406SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Potassium-transporting ATPase C chain (kdpC), partial CSB-YP477407SWN1
CSB-EP477407SWN1
CSB-BP477407SWN1
CSB-MP477407SWN1
CSB-EP477407SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L25 (rplY) CSB-YP477408SWN
CSB-EP477408SWN
CSB-BP477408SWN
CSB-MP477408SWN
CSB-EP477408SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP477409SWN
CSB-EP477409SWN
CSB-BP477409SWN
CSB-MP477409SWN
CSB-EP477409SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP477410SWN1
CSB-EP477410SWN1
CSB-BP477410SWN1
CSB-MP477410SWN1
CSB-EP477410SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP477411SWN
CSB-EP477411SWN
CSB-BP477411SWN
CSB-MP477411SWN
CSB-EP477411SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Der GTPase-activating protein YihI (yihI) CSB-YP477412SWN
CSB-EP477412SWN
CSB-BP477412SWN
CSB-MP477412SWN
CSB-EP477412SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 8-amino-7-oxononanoate synthase (bioF) CSB-YP477413SWN
CSB-EP477413SWN
CSB-BP477413SWN
CSB-MP477413SWN
CSB-EP477413SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin o-succinylbenzoate synthase (menC) CSB-YP477414SWN
CSB-EP477414SWN
CSB-BP477414SWN
CSB-MP477414SWN
CSB-EP477414SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP477415SWN
CSB-EP477415SWN
CSB-BP477415SWN
CSB-MP477415SWN
CSB-EP477415SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Rhamnulokinase (rhaB) CSB-YP477416SWN
CSB-EP477416SWN
CSB-BP477416SWN
CSB-MP477416SWN
CSB-EP477416SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Cell division protein ZapB (zapB) CSB-YP477417SWN
CSB-EP477417SWN
CSB-BP477417SWN
CSB-MP477417SWN
CSB-EP477417SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Met repressor (metJ) CSB-YP477418SWN
CSB-EP477418SWN
CSB-BP477418SWN
CSB-MP477418SWN
CSB-EP477418SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin tRNA (uracil (54)-C (5))-methyltransferase CSB-YP477419SWN
CSB-EP477419SWN
CSB-BP477419SWN
CSB-MP477419SWN
CSB-EP477419SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP477420SWN
CSB-EP477420SWN
CSB-BP477420SWN
CSB-MP477420SWN
CSB-EP477420SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Cytidylate kinase (cmk) CSB-YP477421SWN
CSB-EP477421SWN
CSB-BP477421SWN
CSB-MP477421SWN
CSB-EP477421SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0214 protein yfeW (yfeW) CSB-YP477422SWN
CSB-EP477422SWN
CSB-BP477422SWN
CSB-MP477422SWN
CSB-EP477422SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L11 (rplK) CSB-YP477423SWN
CSB-EP477423SWN
CSB-BP477423SWN
CSB-MP477423SWN
CSB-EP477423SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP477424SWN
CSB-EP477424SWN
CSB-BP477424SWN
CSB-MP477424SWN
CSB-EP477424SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Macrodomain Ter protein (matP) CSB-YP477425SWN
CSB-EP477425SWN
CSB-BP477425SWN
CSB-MP477425SWN
CSB-EP477425SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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